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Invitrogen
VPS54 Polyclonal Antibody
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VPS54 Antibody (PA5-89086) in WB
Western blot analysis of VPS54 in extracts of various cell lines. Samples were incubated with VPS54 Polyclonal antibody (Product # PA5-89086) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
产品信息
PA5-89086
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 420-720 of human VPS54 (NP_0010057391)
偶联物
形式
Liquid
浓度
1.95 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: VVKLADQMRM LNFPQWFDLL KDIFSKFTIF LQRVKATLNI IHSVVLSVLD KNQRTRELEE ISQQKNAAKD NSLDTEVAYL IHEGMFISDA FGEGELTPIA VDTTSQRNAS PNSEPCSSDS VSEPECTTDS SSSKEHTSSS AIPGGVDIMV SEDMKLTDSE LGKLANNIQE LLYSASDICH DRAVKFLMSR AKDGFLEKLN SMEFITLSRL METFILDTEQ ICGRKSTSLL GALQSQAIKF VNRFHEERKT KLSLLLDNER WKQADVPAEF QDLVDSLSDG KIALPEKKSG ATEERKPAEV L; Positive Samples: 293T, Mouse kidney, Mouse lung; Cellular Location: Golgi apparatus, trans-Golgi network
靶标信息
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Hepatocellular carcinoma protein 8; protein phosphatase 1, regulatory subunit 164; Tumor antigen HOM-HCC-8; Tumor antigen SLP-8p; Tumor antigen SLP-8p homolog; vacuolar protein sorting 54 homolog; Vacuolar protein sorting-associated protein 54; VPS54 GARP complex subunit
基因别名: HCC8; hVps54L; mSLP8; PPP1R164; SLP-8p; VPS54; VPS54L; WR
UniProt ID: (Human) Q9P1Q0, (Mouse) Q5SPW0
Entrez Gene ID: (Human) 51542, (Mouse) 245944
