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Invitrogen
JMJD1A Polyclonal Antibody
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图: 1 / 5
JMJD1A Antibody (PA5-88279) in ICC/IF
Immunofluorescence analysis of JMJD1A was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KDM3A Polyclonal Antibody (Product # PA5-88279) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-88279
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 975-1283 of human KDM3A (NP_0609032)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.88 mg/mL
规格
88 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Samples: 293T; Cellular Location: Cytoplasm, Nucleus
Immunogen sequence: PVMVSGVHHK LNSELWKPES FRKEFGEQEV DLVNCRTNEI ITGATVGDFW DGFEDVPNRL KNEKEPMVLK LKDWPPGEDF RDMMPSRFDD LMANIPLPEY TRRDGKLNLA SRLPNYFVRP DLGPKMYNAY GLITPEDRKY GTTNLHLDVS DAANVMVYVG IPKGQCEQEE EVLKTIQDGD SDELTIKRFI EGKEKPGALW HIYAAKDTEK IREFLKKVSE EQGQENPADH DPIHDQSWYL DRSLRKRLHQ EYGVQGWAIV QFLGDVVFIP AGAPHQVHNL YSCIKVAEDF VSPEHVKHCF WLTQEFRYL
靶标信息
JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp686A24246; DKFZp686P07111
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