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Invitrogen
MED8 Polyclonal Antibody
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MED8 Antibody (PA5-79660) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of MED8 in U87 cells using MED8 Polyclonal Antibody (Product # PA5-79660). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL and mouse anti-Tubulin beta antibody overnight at 4°C. Cy3 conjugated goat anti-rabbit IgG and DyLight 488 conjugated goat anti-mouse IgG were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visuali... View More
产品信息
PA5-79660
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human MED8 recombinant protein (Position: M1-R195).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human THP-1 whole cell, human HepG2 whole cell, human U251 whole cell, human Caco-2 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue. IHC: mouse testis tissue, rat testis tissue. ICC/IF: U87 cell. Flow: 293T cells.
靶标信息
This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activator-recruited cofactor 32 kDa component; ARC32; Mediator complex subunit 8; Mediator of RNA polymerase II transcription subunit 8; mediator of RNA polymerase II transcription subunit MED8; mediator of RNA polymerase II transcription, subunit 8 homolog; MGC17544; MGC19641
基因别名: 2210021A15Rik; AB041805; ARC32; MED8; MNCb-2386
UniProt ID: (Human) Q96G25, (Mouse) Q9D7W5
Entrez Gene ID: (Human) 112950, (Mouse) 80509, (Rat) 362575
