Proteintech

RAP1GAP Polyclonal Antibody, CoraLite® Plus 488

Name: RAP1GAP Polyclonal Antibody, CoraLite\u00AE Plus 488
Brand: Proteintech
SKU: CL488-19174

查看其他15个RAP1GAP抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

RAP1GAP Antibody in Immunocytochemistry (ICC/IF)

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RAP1GAP Antibody (CL488-19174) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RAP1GAP antibody (CL488-19174) at dilution of 1:200.

RAP1GAP Antibody in Flow Cytometry (Flow)

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产品信息

CL488-19174

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:500

流式细胞分析 (Flow)

0.80 µg/10^6 cells in a 100 µL suspension

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

RAP1GAP fusion protein Ag5576

偶联物

CoraLite® Plus 488 CoraLite® Plus 488 CoraLite® Plus 488

激发/发射光谱

493/522 nm

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.5% BSA

内含物

0.05% ProClin 300

保存条件

-20°C, store in dark

运输条件

Wet ice

产品详细信息

Immunogen sequence: FLHAYVVVQ AEGGGPDGPL YKVSVTARDD VPFFGPPLPD PAVFRKGPEF QEFLLTKLIN AEYACYKAEK FAKLEERTRA ALLETLYEEL HIHSQSMMGL GGDEDKMENG SGGGGFFESF KRVIRSRSQS MDAMGLSNKK PNTVSTSHSG SFAPNNPDLA KAAGISLIVP GKSPTRKKSG PFGSRRSSAI GIENIQEVQE KRESPPAGQK TPDSGHVSQE PKSENSSTQS SPEMPTTKNR AETAAQRAEA LKDFSRSSSS ASSFASVVEE TEGVDGEDTG LESVSSSGTP HKRDSFIYST WLEDSVSTTS GGSSPGPSRS PHPDAGKLGD PACPEIKIQL EASEQHMPQL GC

靶标信息

Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.

仅用于科研。不用于诊断过程。未经明确授权不得转售。