OriGene

RGMA Monoclonal Antibody (OTI4G8), TrueMAB™

Name: RGMA Monoclonal Antibody (OTI4G8), TrueMAB\u2122
Brand: OriGene
SKU: CF811560

查看其他15个RGMA抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

RGMA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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RGMA Antibody (CF811560) in IHC (P)

Immunohistochemical staining of paraffin-embedded human embryonic cerebellum within the normal limits using anti-RGMA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA811560)(1:250)

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Immunogen image

Immunogen Image RGMA Monoclonal Antibody (OTI4G8), TrueMAB™

产品信息

CF811560

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:250

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

OTI4G8

抗原

Human recombinant protein fragment corresponding to amino acids 169-424 of human RGMA produced in E.coli.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 8% trehalose

内含物

no preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

靶标信息

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

仅用于科研。不用于诊断过程。未经明确授权不得转售。