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Invitrogen
ULK1 Polyclonal Antibody
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ULK1 Antibody (PA5-15378) in ICC/IF
Immunofluorescent analysis of U251 cells stained with an APG1 polyclonal antibody (Product # PA5-15378). U251 cells were treated with Chloroquine (50 uM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), cells were then incubated with an APG1 polyclonal antibody (Product # PA5-15378) (1:100, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min).
产品信息
PA5-15378
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide between 642-672 amino acids from the central region of human APG1 (ULK1)
偶联物
形式
Liquid
浓度
Lot-specific
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
靶标信息
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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