Affinity Chromatography Resins

On this page:

Promote efficient purification with affinity chromatography resins

Achieving high purity and yield during biologics purification can be challenging with the myriad of therapeutic formats. Within Thermo Fisher Scientific’s connected bioprocessing workflows, affinity chromatography resins offer a selective, single-step solution designed to simplify recovery and support reproducible performance. CaptureSelect technology supports affinity chromatography for advanced antibody formats including bispecifics and multispecifics, Fabs and fragments, as well as recombinant proteins and viral vectors.

Benefits of affinity chromatography resins

Affinity chromatography resins enable efficient, high-yield purification under gentle conditions that help preserve quality and reduce downstream polishing. Integrated into bioprocessing workflows, the resins allow scalable performance from process development through cGMP manufacturing. Some benefits of CaptureSelect resins include:

  • High specificity for a particular epitope of the target molecule
  • High binding capacity for concentrated feedstreams
  • Mild elution conditions for pH-sensitive proteins
  • Alternative when Protein A (ProA) chromatography does not meet process goals

Plasma fractionation

Plasma fractionation separates plasma proteins—such as albumin, immunoglobulins, and clotting factors—for various therapeutic applications. Chromatography steps integrated into these workflows facilitate selectivity and consistency, for improved overall yield of precious plasma-derived products. Thermo Fisher supports improved productivity in plasma purification processes with affinity and polishing resins implemented at many stages.
 

Affinity chromatography resins for both cGMP manufacturing and bench or research scale

While the CaptureSelect affinity resins are ultimately designed and used in bioprocess development and manufacturing, our portfolio supports researchers with comprehensive solutions for high-specificity purification at the bench. Covering additional antibodies, proteins, fragments, hormones, enzymes, and viral targets, these resins enable efficient and reproducible isolation of biomolecules to accelerate discovery and development.

Product information

Click the product name for ordering information.

Antibody therapeutics

Product

Characteristics
CaptureSelect IgG-Fc (ms)
  • Designed to purify IgG from multiple species by high affinity binding to the Fc region of IgGs
  • Magnetic bead technology: IgG-Fc (ms)
CaptureSelect IgG-Fc (rabbit)
  • Designed to purify rabbit IgG from recombinant and plasma sources
CaptureSelect CH1-XL
  • Fab fragment purification platform (all Fab κ/λ)
  • No binding of free light chains
  • Efficient elution at milder pH
CaptureSelect FcXP
  • High dynamic binding capacity
  • Efficient elusion at milder pH
POROS CaptureSelect FcXP
  • High dynamic binding capacity
  • Efficient elution at milder pH
  • Efficient purification of larger biomolecules
POROS CaptureSelect ClusterinClear
  • High dynamic binding capacity
  • High selectivity
  • Efficient purification of larger biomolecules
MabCaptureC
  • High-performance Protein A chromatography resin for capturing mAbs and Fc fusion proteins with cost of goods in mind

Product

Characteristics
CaptureSelect LC-kappa (mur)
  • Recognizes IgG, IgM, IgA, IgD, IgE, Fab fragments, Poly-Ig from mouse, rat, and guinea pig
CaptureSelect LC-lambda (mouse)
  • Recognizes IgG, IgM, IgA, IgD, IgE, Fab fragments containing a lambda light chain
CaptureSelect KappaXP
  • High dynamic binding capacity
  • Efficient elution at milder pH
  • Suitable for bispecific antibodies
CaptureSelect LambdaXP
  • High dynamic binding capacity
  • Efficient elution at milder pH
  • Suitable for bispecific antibodies

Product

Characteristics
POROS CaptureSelect IgM-XL Suitable for the purification of human, mouse, and rat IgM
CaptureSelect IgA-CH1 (Hu) Designed for the purification of human IgA, IgA dimers, secretory IgA (sIgA), and Fab fragments
CaptureSelect Bovine IgA Enables purification of monomeric, dimeric, and secretory bovine IgA from whey
CaptureSelect IgE Designed for the purification of human IgE from recombinant and plasma sources

Product

Characteristics
CaptureSelect IgG4 (Hu) Specific for human IgG4 without cross-binding to other subclasses or species
CaptureSelect IgG1 (Hu) Specific for human IgG1 without cross-binding to other subclasses or species
CaptureSelect IgG3 (Hu) Specific for human IgG3 without cross-binding to other subclasses or species

Resin

Characteristics

Ligand Leakage ELISA
CaptureSelect CH1-XL
  • Fab fragment purification platform (all Fab κ/λ)
  • No binding of free light chains
  • Efficient elution at milder pH
 CaptureSelect CH1-XL Ligand Leakage ELISA
CaptureSelect KappaXP
  • High dynamic binding capacity
  • Efficient elution at milder pH
  • Suitable for bispecific antibodies
 CaptureSelect KappaXP Ligand Leakage ELISA
CaptureSelect LambdaXP
  • High dynamic binding capacity
  • Efficient elution at milder pH
  • Suitable for bispecific antibodies
 CaptureSelect LambdaXP Ligand Leakage ELISA
CaptureSelect FcXP
  • High dynamic binding capacity
  • Efficient elusion at milder pH
 CaptureSelect FcXP Ligand Leakage ELISA
POROS CaptureSelect FcXP
  • High dynamic binding capacity
  • Efficient elution at milder pH
  • Efficient purification of larger biomolecules
 CaptureSelect FcXP Ligand Leakage ELISA
POROS CaptureSelect ClusterinClear
  • High dynamic binding capacity
  • High selectivity
  • Efficient purification of larger biomolecules
 POROS CaptureSelect ClusterinClear Ligand Leakage ELISA

Recombinant proteins and biosimilars

Resin

Characteristics
CaptureSelect EPO Designed for the purification of erythropoietin (EPO) and darbepoetin (DPO)
POROS CaptureSelect AdV5 Suitable for the purification and/or scavenging of recombinant hexon capsomeres of adenovirus serotype 5 (AdV5) particles and free hexon proteins
CaptureSelect Multi-Species Albumin Depletion Product Designed for the depletion of albumin from plasma or serum derived from multiple species, such as mouse, rat, rabbit, goat, bovine, guinea pig, human, and horse
CaptureSelect Factor II(a) Suitable for the purification of plasma and recombinant Factor II and Factor II(a)
CaptureSelect Factor H Designed for the purification of human complement Factor H from plasma and recombinant sources
POROS CaptureSelect Fib XL Suitable for the purification of plasma-derived and recombinant fibrinogen
CaptureSelect Transferrin Suitable for the purification of plasma-derived and recombinant transferrin
CaptureSelect Prothrombin Designed for the purification of human prothrombin (coagulation Factor II)
CaptureSelect C-tagXL Designed for the purification of C-terminal tagged proteins containing the four-amino acid peptide tag (E-P-E-A) with high affinity and selectivity from complex mixtures, such as cell culture harvests and periplasmic fractions
CaptureSelect FSH Suitable for binding intact follicle-stimulating hormone (FSH) at the alpha/beta chain interface
CaptureSelect Human Albumin Designed to support the gentle elution of albumin fusion proteins with mild elution properties
CaptureSelect hCG Suitable for binding human chorionic gonadotropin (hCG) without cross-reactivity to other gonadotropins, such as FSH, LH, and TSH
CaptureSelect Human Growth Hormone Designed for the selective purification of human growth hormone (hGH) with retained activity of the protein
CaptureSelect Factor X Suitable for binding Factor X and Factor Xa in the presence of calcium enables efficient purification with retained activity of the protein without cross-binding to other plasma proteins
CaptureSelect tPA Designed for binding tissue plasminogen activator (tPA) in the presence of magnesium chloride at neutral pH
CaptureSelect TSH Suitable for binding thyroid-stimulating hormone (TSH) without cross-reactivity to other gonadotropins, such as FSH, LH, and hCG
CaptureSelect AAT-XL Designed for the purification of plasma-derived and recombinant alpha-1 antitrypsin (AAT)
MabCaptureC High-performance Protein A chromatography resin for capturing mAbs and Fc fusion proteins with cost of goods in mind

Resin

Characteristics

Ligand Leakage ELISA
CaptureSelect C-tagXL Designed for the purification of C-terminal tagged proteins containing the four-amino acid peptide tag (E-P-E-A) with high affinity and selectivity from complex mixtures, such as cell culture harvests and periplasmic fractions CaptureSelect C-tagXL Ligand Leakage ELISA
CaptureSelect FSH Suitable for binding intact follicle-stimulating hormone (FSH) at the alpha/beta chain interface CaptureSelect FSH Ligand Leakage ELISA
CaptureSelect Human Albumin Designed to support the gentle elution of albumin fusion proteins with mild elution properties CaptureSelect HSA Ligand Leakage ELISA
CaptureSelect hCG Suitable for binding human chorionic gonadotropin (hCG) without cross-reactivity to other gonadotropins, such as FSH, LH, and TSH CaptureSelect hCG Ligand Leakage ELISA
CaptureSelect Human Growth Hormone Designed for the selective purification of human growth hormone (hGH) with retained activity of the protein CaptureSelect hGH Ligand Leakage ELISA
CaptureSelect Factor X Suitable for binding Factor X and Factor Xa in the presence of calcium enables efficient purification with retained activity of the protein without cross-binding to other plasma proteins CaptureSelect Factor X Ligand Leakage ELISA
CaptureSelect tPA Designed for binding tissue plasminogen activator (tPA) in the presence of magnesium chloride at neutral pH CaptureSelect tPA Ligand Leakage ELISA
CaptureSelect TSH Suitable for binding thyroid-stimulating hormone (TSH) without cross-reactivity to other gonadotropins, such as FSH, LH, and hCG CaptureSelect TSH Ligand Leakage ELISA
CaptureSelect AAT-XL Designed for the purification of plasma-derived and recombinant alpha-1 antitrypsin (AAT) CaptureSelect AAT-XL Ligand Leakage ELISA

Gene therapy purification

Resin

Characteristics
POROS CaptureSelect AdV5 Suitable for the purification and/or scavenging of recombinant hexon capsomeres of AdV5 particles and free hexon proteins
POROS CaptureSelect BacuClear  Designed for the reduction of baculovirus contaminants when using baculovirus expression systems (BEVS) to produce recombinant biomolecules
CaptureSelect Lenti VSVG Designed for the purification of vesicular stomatitis virus G protein (VSV-G) lentivirus particles
Dynabeads CaptureSelect AAVX Suitable for the purification of a broad range of naturally occurring and synthetic AAV serotypes
Dynabeads CaptureSelect AAV9 Designed for the purification of naturally occurring and synthetic adeno-associated virus type 9 (AAV9) serotypes
GoPure AAVX Affinity Membrane Designed to offer rapid capture of AAV vectors at a small scale
Exosomes CD81 Designed for the affinity capture of human extracellular vesicles (EVs) like exosomes by binding to the tetraspanin surface protein CD81
POROS CaptureSelect AAVX Platformable pan-serotropic affinity resin for the capture of most AAV serotypes, including chimeric / synthetic serotypes, with a high binding capacity of > 10^14 vg/mL
POROS CaptureSelect AAV9 Affinity resin for the capture of AAV9 serotype, with a high binding capacity of > 10^14 vg/mL
POROS CaptureSelect AAV8 Affinity resin for the capture of AAV8 serotype, with a high binding capacity of > 10^13 vg/mL

Resin

Binding capacity (vg/mL)*

Serotype affinity

Ligand Leakage ELISA
POROS CaptureSelect AAVX >1014 AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, recombinant and chimeric vectors** CaptureSelect AAVX
POROS CaptureSelect AAV9 >1014 AAV9 CaptureSelect AAV9
POROS CaptureSelect AAV8 >1013 AAV8 CaptureSelect AAV8


* Measured as viral genomes per milliliter (vg/mL)

** The AAVX affinity ligand has demonstrated binding reactivity towards a large set of AAV serotypes (including chimeric vectors), suggesting that an even broader range of AAV serotypes might be purified by this resin.

mRNA purification

Resin

Characteristics
POROS Oligo (dT)25 Designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes

Resin

Characteristics
POROS Oligo (dT)25 Designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes

Other modalities and applications supported

ADCs

Antibody-Drug Conjugates (ADCs) workflows rely on chromatography steps to separate complex conjugates and reduce impurities. These approaches support consistent production of ADCs for therapeutic research and biomanufacturing.
 

Cell Therapy

Cell therapies utilize living cells as treatments, necessitating strict control over inputs and processing steps. Chromatography is applied to prepare ancillary materials, such as cytokines or viral vectors, that are used during cell manufacturing.
 

Gene Therapy

Gene therapy often relies on viral vectors, such as adeno-associated virus (AAV) or lentivirus, to facilitate the transfer of genetic material. Chromatography separates drug product from product- and process-related impurities in both capture and polishing steps.

mAbs

Monoclonal antibody purification typically starts with Protein A chromatography to capture the antibody from cell culture fluid. Additional steps, such as ion exchange, are used to help reduce aggregates, variants, or process-related impurities.
 

mRNA

Chromatography in mRNA production separates full-length transcripts from double-stranded RNA and truncated products. The purified RNA can then be used in formulation or downstream steps, such as lipid nanoparticle assembly.
 

Vaccines

Chromatography is often used in vaccine manufacturing for both the capture and polishing of the vaccine drug product. The choice of method depends on the platform, for example, protein subunits, viral vectors, or nucleic acid vaccines.
 

Frequently asked questions

Resins for affinity chromatography are used to purify biomolecules based on specific interactions between a ligand and its target. They allow the selective capture of antibodies, fragments, recombinant proteins, and viral vectors in a single purification step. This specificity helps minimize the need for additional polishing steps, thereby helping improve process yield, recovery, and overall efficiency from development through cGMP manufacturing.

Resin selection depends on the molecule’s size, structure, and intended purification conditions. Consider ligand specificity, binding strength, and elution requirements when comparing options. Thermo Fisher offers custom resin development services for projects that require specific ligand chemistries or performance characteristics. Learn more about custom resin development or custom POROS development.

Affinity chromatography resins are produced under controlled quality systems and can be used in cGMP manufacturing. Each batch is released with supporting documentation, including certificates of analysis and regulatory files, for enhanced traceability.

The chromatography product portfolio includes anion exchange chromatography (AEX) and hydrophobic interaction chromatography (HIC) resins for different stages of purification. Chromatography columns and pre-packed formats are available to support method setup and scale-up. 96-well screening plates enable rapid resin evaluation by allowing multiple purifications to run in parallel.

Affinity chromatography is commonly used as one of the first capture steps in monoclonal antibody purification. Protein A resins bind the Fc region of antibodies, allowing selective recovery from cell culture fluid. Subsequent steps, such as ion exchange and polishing, reduce aggregates and host-cell proteins.

Evaluation samples are available for small-scale testing and process development. These allow comparison of binding capacity, recovery, and elution behavior before scaling up. Requests can be submitted through the Biotherapeutics Purification Sample Request Form.

Explore other chromatography resins

Anion exchange chromatography resins

AEX resins capture negatively charged species through interactions with positively charged functional groups. Strong AEX resins maintain capacity across a wide pH and conductivity range for both capture and polishing.


 

Cation exchange chromatography resins

Cation exchange chromatography (CEX) resins separate positively charged biomolecules using negatively charged groups on the matrix. This approach is often used to purify proteins, peptides, and enzymes, offering stable performance from process development through cGMP manufacturing.

Hydrophobic interaction chromatography resins

HIC separates biomolecules by surface hydrophobicity. HIC is commonly used as a polishing step to improve purity when charge-based methods are limited.



 

Ion exchange chromatography resins

Ion exchange chromatography (IEX) resins rely on charge interactions with functional groups to separate biomolecules. Available in strong and weak base or acid chemistries, they are suited for bind/elute or flow-through purification of antibodies, vaccines, viral vectors, and other biologics.

Mixed-mode chromatography resin

Mixed-mode chromatography uses ion exchange and hydrophobic interactions within a single chemistry to improve selectivity in complex samples. POROS mixed-mode cation exchange chromatography resin is used to reduce aggregates, host cell proteins, and other impurities.

Protein A chromatography resin

Protein A chromatography is widely used for antibody purification. The MabCaptureC resin offers high binding capacity, alkaline stability, and scalability, while keeping costs of goods in mind, making it suitable for process development and cGMP manufacturing.
 

Explore more chromatography resins

Chromatography resins include affinity, IEX, HIC, and mixed-mode formats. They enable reproducible performance from screening tools to cGMP production, supporting process consistency across purification steps.

Not sure which resins are right for you?


Selecting the right resin depends on your molecule type, process stage, and purification goals. Compare chemistries, binding capacities, and scalability data in one place.
 

Custom chromatography resins engineered for purification

Thermo Fisher Scientific collaborates with teams to develop custom chromatography resins that address complex purification challenges. Through custom resin and ligand development, we help bridge workflow gaps, refine selectivity, and enhance performance from process design through cGMP production.

Chromatography resources


Access resources that cover chromatography performance, purification strategies, and method development. These resources empower scientists to select and apply chromatography resins from research to cGMP manufacturing.

Let us help you start your purification journey

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.