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The second episode of the 2026 Back to Basics series provides a comprehensive overview of flow cytometric crossmatch (FCXM) testing in HLA laboratories. The session covers the scientific principles of flow cytometry and how they are applied to crossmatch testing, including key steps in the FCXM workflow. It highlights the sensitivity of this method in evaluating potential donor-specific antibodies and its role in supporting donor-recipient compatibility assessment. The presentation also discusses result interpretation and clinical relevance, and its use in current transplantation practices.
Instructor:
Linda Olsen, MPH, CHS(ACHI), MLS(ASCP)CM,
Sr. Technical Training Specialist – Molecular, Thermo Fisher Scientific
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