Flow Cytometry for HLA. Back to Basics Series, Episode 4

Flow Cytometry for HLA, Episode 4

This presentation provides an overview of flow cytometry principles and their application to HLA testing in clinical and transplant settings. It begins with a review of how flow cytometers function. The immunophenotyping section explains the use of fluorophore-conjugated antibodies and CD markers to identify immune cell populations, with examples of diagnostic and monitoring applications. A portion focuses on flow cytometric crossmatch (FCXM), briefly describing its workflow, interpretation, and clinical value compared to complement-dependent cytotoxicity crossmatch. The presentation concludes with an introduction to other HLA flow-based technologies, including FlowPRA and Flow-DSA XM, highlighting their roles, advantages, and limitations in antibody detection and transplant risk assessment.

Instructors:
Linda Olsen, MPH, CHS(ACHI), MLS(ASCP)^CM,
Sr. Technical Training Specialist – Molecular, Thermo Fisher Scientific

HLA & Transplant Back to Basics Series ⠀⠀ ⠀

Watch the series

Meet the speaker

Linda serves as Technical Training Specialist for our molecular products, a role she has held since March 2022. She holds a bachelor’s degree in clinical laboratory science and a master’s in public health policy and management. She has worked in HLA since 2008, with experience at the University of Utah, Stanford, and ClinImmune Labs. Prior to joining the Technical Training team, she was supervisor of the HLA lab at Mayo Clinic Arizona. She has also served as an ASHI inspector and committee member. She enjoys teaching and increasing access to educational materials on the science and application of HLA.

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