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Leading the future of flow cytometry and innovative solutions: please visit us at booth #301
Discover. Connect. Innovate.
Join us at CYTO 2025 to explore innovations in flow cytometry, imaging, and cell sorting. Whether you're looking to solve specific research challenges, streamline workflows, or experience the power of our latest technologies firsthand, this is your chance to engage directly with Thermo Fisher Scientific specialists.
Book your free one-on-one specialist session
Limited spots available
Take your research further with a personalized consultation or live demo. Whether you’re navigating complex challenges, optimizing your flow cytometry panel design, or exploring the latest in spectral technology, we’ll connect you with the right specialist—R&D scientists, software developers, hardware engineers, or panel design specialists.
Sessions are just 30 minutes—quick, impactful, and tailored to you.
Spots are limited and filling fast—reserve yours today!
- Tutorial session
- Posters
- New innovations
Don’t miss our tutorial sessions
Tuesday, June 3 | 12:15–1:15 PM | Mile High Ballroom 1A-C
Enhancing Cell Sorting with Imaging-Enabled Cytometry
Speakers: Nate Trujillo, PhD & Mike Ward, PhD
Abstract
The integration of advanced imaging cytometry with traditional cell sorting techniques offers a transformative approach to cellular analysis and sorting. In this presentation, we explore the synergistic use of the Invitrogen Bigfoot Spectral Cell Sorter and the Invitrogen Attune CytPix Flow Cytometer, an imaging-enabled flow cytometer, to enhance the precision and reliability of cell sorting processes. By leveraging the high-resolution imaging capabilities and additional morphological parameters of the Attune CytPix Flow Cytometer, a pre-sort assessment can be performed to evaluate cell quality, refine gating strategies, and help ensure proper populations are sorted to improve overall efficiency and accuracy. After sorting, the Attune CytPix platform can be used to verify the purity and composition of the sorted cell populations. This step is essential for verifying the success of the sorting protocol and helping ensure that the intended cell subset has been accurately isolated.
This study also investigated employment of the Attune CytPix platform to perform automated morphology analysis of acute myeloid leukemia (AML) samples. With a new, exceptional machine learning model, the Attune CytPix Flow Cytometer brings the benefits of brightfield imaging to introduce new morphological parameters and high dimensional tools such as uMAP for added data visualization and analysis. Using this technology, a cell is selected, and the software automatically searches for similar cells. From this data, we found that less than 1% of the sample showed cell binding between hyperdiploid leukemia cells and white blood cells. This methodology facilitates the detection of rare hyperdiploid cells, and their interactions with white blood cells—a phenomenon that reduces detection of leukemia cells and thwarts therapeutic efforts. The ability to discern different AML phenotypes based on morphology can open new avenues for research and therapeutic interventions, helping advance the field of leukemia research and treatment.
Wednesday, June 4 | 12:15–1:15 PM | Mile High Ballroom 1D
Boosting Core Facility Efficiency with Bigfoot & Attune Xenith
Speakers: Rachael Walker, PhD & William Kang
Abstract
Flow cytometry core facilities play a pivotal role in advancing research, requiring state-of-the-art tools to meet the demands of high-throughput and sometimes complex analyses. This talk will focus on the transformative impact of the Thermo Fisher Bigfoot Cell Sorter and Attune Xenith Flow Cytometer within these facilities. The Thermo Fisher Bigfoot Cell Sorter is a game-changer, offering exceptional sorting speed and precision, which significantly reduces processing times and increases sample throughput. The Attune Xenith Flow Cytometer, with its advanced spectral flow cytometry capabilities and acoustic focusing, allows for the simultaneous detection of multiple fluorochromes with high resolution and accuracy. These instruments not only enhance the analytical capabilities of core facilities but also provide substantial time savings, enabling more efficient workflow management and resource utilization. By integrating the Bigfoot Cell Sorter and Attune Xenith Flow Cytometer, at Babraham Institute, we have been able to achieve new levels of performance and productivity, ultimately driving forward scientific discovery and innovation.
Visit our scientific posters
Monday, June 2, 2025, 5:00–6:30pm
Board P104
Brandon Trent
Expansive immunophenotyping of natural killer cell populations with the Invitrogen Attune Xenith Flow Cytometer
Board P191
Yongfen Chen
Advanced single-color controls for better spectral flow cytometry unmixing
Board P221
Susan O’Meara
Enhancing cell recovery with ultra-recovery mode in Bigfoot Spectral Sorter
Board P251
William Kang/Seddon Thomas
Block party: Solutions to reduce panel background and expand dye selection
Tuesday, June 3, 2025, 5:00–6:30pm
Board P050
Nicole Madfis
Advanced acute myeloid leukemia research: leveraging automated morphology analysis in flow cytometry to identify rare hyperdiploid cell interactions
Board P068
Jasmine Padilla
Predictive modeling of T cell expansion: leveraging morphological metrics with Invitrogen Attune CytPix Flow Cytometer for enhanced cell therapy manufacturing
Board P139
William Kang
A predictive toolkit for evaluating single-color controls in spectral flow cytometry
William Kang and Jayanth Narayanan
Board P202
Adam York
ERF bead calibrators and custom 1:1 antibody:dye labeling to improve antibodies bound per cell calculations
Board P216
Thiago Alves da Costa
Accelerating high-parameter flow cytometry: enhancing analysis time with rapid data acquisition
Board P222
Matt Meyer
Enhancing cell sorting efficiency through optimized forward scatter resolution
Board P222
Mitch Klomp
Impact of tip size on side stream stability in cell sorting
Board P284
Bethan Jones
Enhancing flow cytometry analysis with user-driven model training and advanced data visualization tools for the image-enhanced Invitrogen Attune CytPix System
Board P013, Electronic Poster (NEW)
Emmanuel Acheampong
Detection of circulating rare cell through an integrated inverse magnetophoresis enrichment method and multiparametric image flow cytometry
Board P191
Yongfen Chen
Advanced unmixing single color controls for better spectral flow cytometry unmixing accuracy
Featured innovations
Explore the latest technologies advancing cell analysis, imaging, and spectral flow cytometry.
From advanced cytometers to next-generation spatial imaging platforms, our latest innovations are engineered to accelerate discovery, simplify workflows, and deliver deeper insights. Whether you're designing high-parameter panels, integrating real-time imaging, or enhancing core lab performance, these solutions bring precision and power directly to your research.
See them live at Booth #301—experience what’s new, and what’s next.
Attune Xenith Flow Cytometer
High-performance spectral flow cytometry with acoustic-assisted focusing for fast, flexible, and reliable assay setup across conventional and spectral modes.
Attune CytPix Flow Cytometer
Integrates high-speed flow cytometry with real-time cellular imaging and AI-driven morphology analysis for deeper, data-rich insights.
Bigfoot Spectral Cell Sorter
High-throughput, high-parameter cell sorting with advanced spectral technology and a compact, ultra-quiet design built for core facility workflows.
EVOS S1000 Spatial Imaging System
Multiplex widefield imaging with intuitive software and single-round 9-plex spectral unmixing for streamlined, high-resolution spatial analysis.
The Invitrogen EVOS S1000 Spatial Imaging System is only available in participating countries and regions including US, Canada, Australia and Europe in 2025.
For Research Use Only. Not for use in diagnostic procedures.