Vanquish Amplify UHPLC Systems

Metal-sensitive biomolecules, oligonucleotides, peptides, and other biotherapeutics can interact with metal surfaces throughout the chromatographic flow path, potentially affecting analyte recovery, peak shape, sensitivity, and reproducibility. Inert UHPLC workflows help you reduce unwanted secondary interactions that may contribute to adsorption, carryover, or inconsistent chromatographic performance while supporting demanding applications such as oligonucleotide analysis, protein characterization, peptide mapping, intact mass analysis, and bioanalysis workflows requiring high analytical sensitivity and reproducibility.

In LC-MS analysis of oligonucleotides, peptides, and other metal-sensitive biomolecules, interactions with exposed metal surfaces can promote the formation of unwanted metal adducts, which complicate spectra, reduce mass accuracy, and lower confidence in analyte identification. Vanquish Amplify UHPLC systems have an inert sample flow path which minimizes metal adduct formation, resulting in cleaner mass spectra, improved data interpretation, and more reliable characterization of complex biotherapeutics.

MS ion suppression can negatively impact analyte response, sensitivity, and quantitative reproducibility, particularly for low-abundance or highly complex biopharmaceutical samples. Optimized inert UHPLC workflows can help reduce secondary interactions and improve analyte transfer into the mass spectrometer, supporting higher MS sensitivity, improved signal consistency, and more confident detection of challenging biomolecules.

In UHPLC applications, inconsistent or improperly tightened fluidic connections can contribute to leaks, dead volume, and reduced chromatographic performance. Thermo Scientific Viper TQ UHPLC fittings help simplify installation through a built-in torque control mechanism ensuring consistent and easy installation that you can hear and feel, preventing leaks due to over- and under-tightened connections.

Biopharmaceutical workflows often involve precious and limited-volume samples where sample loss can directly affect analytical sensitivity and reproducibility. Advanced vial bottom detection capabilities help maximize sample utilization and support consistent injection precision during low-volume analytical workflows.