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The Analyst, FlexStation and SpectraMax M5/M5e from Molecular Devices have received the LanthaScreen Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.

Download Terbium assay application note

Download Europium assay application note

SpectraMax Paradigm Microplate Detection Platform

 

Instrument Configuration:

Assay Technology Adapta &
LanthaScreen
Europium
GeneBLAzerLanthaScreen
Terbium
PredictorZ'-LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide
Application Note
Poster--
Excitation Filter 406/15 532/25 
Emission 1 Filter 465/35 595/35  
Emission 2 Filter 535/25 595/35  
Dichroic Mirror 508   
Optic Module GeneBLAzer FP rhodamine  

 

SpectraMax M5/M5e Microplate Reader
& FlexStation 3 Benchtop Multi-Mode Microplate Reader


 
 

 

Instrument Configuration:

Assay Technology Adapta &
LanthaScreen
Europium
GeneBLAzerLanthaScreen
Terbium
PredictorZ'-LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

Reader not
compatible

Application Note-----
Poster-----
Excitation332/12410/12332/12 410/12
Emission 1620/12458/12488/12 458/12
Emission 2666/12522/12518/12 522/12
Dichroic Mirror590/630435/495420 435/495

FilterMax F5 Multi-Mode Microplate Reader

 

Instrument Configuration:

Assay Technology Adapta &
LanthaScreen
Europium
GeneBLAzerLanthaScreen
Terbium
PredictorZ'-LYTE
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup GuideReader not compatibleReader not compatibleReader not compatible
Application Note
Poster--
Excitation Filter 405/30   405/30
Emission 1 Filter 465/35   465/35
Emission 2 Filter 535/25   535/25
Dichroic Mirror     

 

Analyst Multimode Reader


   

 

Instrument Configuration:

Assay Technology Adapta &
LanthaScreen
Europium
GeneBLAzerLanthaScreen
Terbium
PredictorZ'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

-

-

 

-

-

Application Note-----
Poster-----
Excitation Filter (25 mm)340/30 (PV00225)405/20340/30 (PV00225)530/25405/20
Emission 1 Filter (25 mm)620/7.5460/40495/10 (PV00325)580/10460/40
Emission 2 Filter (25 mm)665/7.5530/25520/25 (PV00325)580/10530/25
Dichroic Mirror380 or
400
425 or
50/50
380 or
400
561425 or
50/50

 

* Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above.

** Some compatible filter sets are available for purchase from Life Technologies.  Click on selected filter sets for more information.

Analyst, SpectraMax and FlexStation are registered trademarks of Molecular Devices.

仅供科研使用,不可用于诊断目的。

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