The Varioskan® Flash from Thermo Scientific has received the LanthaScreen® Certified designation from Life Technologies which ensures that this reader is validated to strict standards in instrument setup and assay performance.
These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.
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* Optics shown as center wavelength/bandwidth (nm); Contact Thermo Scientific for information on detection modalities suitable with the microplate reader listed above.
Varioskan® is a registered trademark of Thermo Scientific.