AccuSEQ Real-Time PCR Detection Software Assay Results

For rapid and accurate mycoplasma detection, AccuSEQ software offers a plug-in analysis module for automated presence/absence calls when using the MycoSEQ mycoplasma detection system.
 

Key features and benefits: 

• Optimized and validated for the MycoSEQ Mycoplasma Detection assay
• Automated detection of PCR inhibition in sample wells
• Automated detection of discriminatory positive control (DPC)
• Robust data analysis parameters for the MycoSEQ Mycoplasma Detection assay
• Flexibility for experiments—call settings may also be modified to meet the specifications of individual laboratories

AccuSEQ software offers factory default templates to facilitate experiment set up for the accurate and sensitive quantitation of host cell DNA impurities in both the purification process and in drug substance samples. 
 

Key features and benefits: 

• Streamlined resDNASEQ experiment setup (using factory default templates) and data reporting 
• Automatic calculations of the resDNASEQ standard curve with curve fitting parameters 
• Accurate quantitation of samples with % CV 
• Computation of key metrics including % recovery, total DNA in pg/mL, and pg DNA/mg protein 
• Flexibility for custom experiments to meet the specifications of individual laboratories 
• Customizable output layout that can be exported to a variety of formats 
• Comprehensive reports which help eliminate the need for Excel sheets for calculations and preserve the audit trail 

Detection of mouse minute virus (MMV) and vesivirus

For rapid and accurate MMV and vesivirus detection, the AccuSEQ Software offers: 

• A robust, optimized, and validated system offering data analysis designed for use with ViralSEQ detection systems
• Automated detection for rapid assessment of PCR inhibition in sample wells
• Analysis options to identify true positives from false positives with discriminatory positive control
• Flexible call settings to help you meet specifications for unique lab requirements

Quantitation of Sf-rhabdovirus with AccuSEQ Software 

The AccuSEQ Software offers factory streamlined experimental set up for the accurate and sensitive quantitation of Sf-rhabodvirus contamination in both the purification process and in drug substance samples. 

Key features and benefits: 

• Streamlined experiment setup with factory default templates for easy data reporting
• Automated calculations to help eliminate the need for spreadsheets and preserve the audit trail
• Comprehensive reports that present key metrics, including % Recovery, Total RNA in pg/mL, and pg RNA/mg Protein
• Flexibility to easily adjust for custom experiments and meet specialized specifications and reporting needs

The custom experiment mode—“Create New”—of the AccuSEQ software enables you to create custom experiment setups. It guides you through the experiment properties, qPCR run method details, and plate setup where there are easy-to-use tools for defining and setting up standards, controls, and unknowns. This custom workflow also supports ViralSEQ MMV, vesivirus, and resDNASEQ Sf9-Baculovirus duplex assays.