Choose iBright imaging systems based on detection method, imaging requirements, and quantitation needs. Compare western blot imaging capabilities, analysis tools, and system configurations to identify the right solution for imaging, quantitation, and documentation.

Explore the western blot workflow


Western blot imaging and analysis with iBright imaging systems

 

Western blot imaging is an important step in protein analysis workflows, enabling researchers to detect, document, and quantify target proteins after electrophoresis and membrane transfer. Whether using chemiluminescent, fluorescent, or colorimetric detection methods, obtaining high-quality images is critical for accurate data interpretation and reproducible results.
 

iBright imaging systems combine image acquisition, analysis software, and quantitation tools in a single platform to support western blot imaging, gel documentation, total protein normalization, and protein quantitation.
 

Whether performing routine western blot analysis or advanced protein expression studies, iBright imaging systems help provide a streamlined solution for imaging, quantitation, and documentation throughout the protein analysis workflow.


Applications and capabilities of iBright imaging systems

Convenient, intuitive interface and workflows

Capture, analyze, and manage images using a 12.1-inch capacitive touchscreen with integrated analysis tools that simplify routine western blot and gel imaging workflows.

Powerful camera technology

Capture crisp, high-quality images using a 9.1 MP imaging system with Smart Exposure technology that automatically determines optimal exposure settings. Smart Range HDR helps visualize both weak and strong protein signals in a single image for reliable quantitation.

Essential imaging modes and specialty applications

Support chemiluminescent, fluorescent, and colorimetric western blot imaging, protein and nucleic acid gel documentation, and specialty applications using five imaging modes in a single platform.

Capture more data with multiplex fluorescent western blotting

Detect and quantify up to four protein targets in a single blot using multiplex fluorescent imaging, helping conserve samples and generate more meaningful comparative data.

Streamlined image analysis

Analyze western blot and gel images using integrated on-instrument, desktop, cloud-based, or secure software. Perform densitometry, molecular weight estimation, quantitation, normalization, and image annotation from a single analysis platform.

21 CFR Part 11-ready support

Support regulated laboratory environments with software tools designed to help manage electronic records, audit trails, and user access controls.


Educational resources

Access resources to help optimize western blot detection strategies, troubleshoot experiments, and improve data quality.

iGlow Gel Documentation System—one-touch gel imaging

Experience simple, efficient imaging and documentation for routine protein and nucleic acid gels
when western blot is not required.

Frequently asked questions

A western blot imaging system is an instrument used to visualize, capture, and analyze protein bands on a membrane following western blot detection. Modern imaging systems can support chemiluminescent, fluorescent, and colorimetric detection methods while providing software tools for image analysis and quantitation.

Western blot quantitation is typically performed using densitometry analysis software that measures band intensity. Researchers can compare protein expression levels across samples and normalize results using total protein stains or housekeeping proteins.

Densitometry is a quantitative image analysis method used to measure the intensity of protein bands in a western blot image. The resulting values can be used to evaluate relative protein abundance and compare expression levels between samples.

Smart Exposure technology automatically identifies optimal image acquisition settings to help reduce trial-and-error adjustments and improve imaging consistency across experiments.

For Research Use Only. Not for use in diagnostic procedures.