SelectScreen Kinase Profiling Services

The Accelerated Data Delivery service returns up to 80% of your kinase profiling data to you in as little as 48 hours. SelectScreen Real-Time Data Delivery enables customers to securely retrieve screening data as it is generated, providing immediate access for downstream analysis, visualization, and decision-making.

• Average data delivery time of just 5 days: so you can get data to your medicinal chemists more quickly
• Access data in real-time as soon as it is available via our secure online portal
• Broad panel of more than 465 purified kinases: help to establish how selective a compound is to its intended targets
• Robust compound management process: helps ensure sample quality and data consistency from project initiation through reporting.
• Complete lab information management system (LIMS): helps ensure project traceability and consistency

Customize your kinase profiling project to get the right data for your research.

Standard service options include:

• Targets: 465 available biochemical assays
• Mode: single point concentrations and IC50 and/or EC50 determinations
• Choice of ATP concentration: 10 µM, 100 µM, and Km [app] ATP (activity assays only)

For the kinases that are available in our activity assays, comprehensive titrations are performed against our panel of available substrates. In addition, ATP titrations are performed for each of these kinases to determine the Km[app] ATP for each target. As a result, you can choose to have your compound run at, above, or below the Km[app] ATP for each kinase within the panel that utilizes an activity assay. This flexibility allows you to identify specific types of inhibitors with various mechanisms of action.

Currently, two different assay platforms are available for profiling: activity and binding. The Z′-LYTE and Adapta kinase activity assays are used most extensively for profiling, and a smaller subset of kinases may be profiled using our LanthaScreen Eu Kinase Binding Assays.

Overview of available formats

A stringent verification process helps ensure quality data, with each data point performed in duplicate.  Strict quality control protocols help ensure that any assay results not meeting set specifications will be automatically repeated, this includes a control inhibitor titration (IC50) for each kinase on every plate.