Rockland

APAF1 Monoclonal Antibody (2E10)

Name: APAF1 Monoclonal Antibody (2E10)
Brand: Rockland
SKU: 200-301-A35

查看其他21个APAF1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

APAF1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

APAF1 Antibody (200-301-A35) in ICC/IF

Immunocytochemistry staining using Rocklands monoclonal anti-Apaf1 antibody at 0.5 µg/mL shows detection of Apaf1 in K562 cells. Cells were fixed in 4% paraformaldehyde.

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Immunogen image

Immunogen Image APAF1 Monoclonal Antibody (2E10)

产品信息

200-301-A35

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200-1:2,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

0.5 µg/mL

酶联免疫吸附实验 (ELISA)

1:10,000-1:40,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG

分类

Monoclonal

类型

Antibody

克隆号

2E10

抗原

Anti-Apaf1 Antibody monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human Apaf1 protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。