Proteintech

CARM1 Polyclonal Antibody

14 篇参考文献

查看其他17个PRMT4抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (12)

CARM1 Antibody in Immunocytochemistry (ICC/IF)

CARM1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 12

CARM1 Antibody (55246-1-AP) in ICC/IF

Immunofluorescent analysis of NIH/3T3 cells using 55246-1-AP (CARM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CARM1 Antibody in Immunocytochemistry (ICC/IF)

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Western Blot (WB)

CARM1 Antibody in Western Blot (WB)

CARM1 Antibody in Western Blot (WB)

CARM1 Antibody in Western Blot (WB)

Product Image Gallery

CARM1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NIH/3T3 cells using 55246-1-AP (CARM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human malignant melanoma tissue slide using (Product # 55246-1-AP) CARM1 antibody at dilution of 1:200 (under 40x lens).

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 55246-1-AP (CARM1 antibody) at dilution of 1:50 (under 40x lens).

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 55246-1-AP (CARM1 antibody) at dilution of 1:50 (under 10x lens).

CARM1 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with (Product # 55246-1-AP) CARM1 antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

CARM1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 55246-1-AP (CARM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CARM1 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55246-1-AP (CARM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CARM1 Antibody in Western Blot (WB) — WB result of CARM1 antibody (55246-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARM1 transfected MCF-7 cells.

产品信息

55246-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:6,000

查看8篇已发表文章 8篇已发表文章

免疫组化 (IHC)

-

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:500

-

免疫细胞化学 (ICC/IF)

1:20-1:200

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

-

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.27 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTM sites in cells themselves.

靶标信息

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Coactivator-associated arginine methyltransferase 1; coactivator-associated arginine methyltransferase 1 variant 5; Histone-arginine methyltransferase CARM1; protein arginine methyltransferase; Protein arginine N-methyltransferase 4

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基因别名: CARM1; PRMT4

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UniProt ID: (Human) Q86X55, (Mouse) Q9WVG6, (Rat) Q4AE70

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Entrez Gene ID: (Human) 10498, (Mouse) 59035, (Rat) 363026

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transcription coactivator activity protein binding beta-catenin binding protein methyltransferase activity histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity ligand-dependent nuclear receptor transcription coactivator activity protein-arginine omega-N asymmetric methyltransferase activity histone methyltransferase activity (H3-R17 specific) histone methyltransferase activity protein homodimerization activity transcription regulatory region DNA binding lysine-acetylated histone binding rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity protein modifying enzyme

regulation of growth plate cartilage chondrocyte proliferation transcription, DNA-templated regulation of transcription, DNA-templated DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest aging positive regulation of cell proliferation viral process histone methylation peptidyl-arginine methylation, to asymmetrical-dimethyl arginine intracellular estrogen receptor signaling pathway negative regulation of protein binding regulation of intracellular estrogen receptor signaling pathway histone H3-R2 methylation histone H3-R17 methylation cellular lipid metabolic process positive regulation of fat cell differentiation response to cAMP endochondral bone morphogenesis regulation of mRNA binding negative regulation of dendrite development protein methylation pathogenesis chromatin modification intracellular steroid hormone receptor signaling pathway methylation histone arginine methylation peptidyl-arginine N-methylation mRNA processing RNA splicing