Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
流式细胞分析 (Flow) |
0.125 µg/test | 查看5篇已发表文章 5篇已发表文章 |
中和实验 (Neu) |
- | 查看2篇已发表文章 2篇已发表文章 |
功能检测 (FN) |
- | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属反应 |
Mouse |
已发表种属 |
Mouse |
宿主/亚型 |
Rat / IgG2a, lambda |
推荐的同型对照 |
Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™ |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
MIH5 |
偶联物 |
Super Bright™ 645
|
激发/发射光谱 |
查看光谱
|
形式 |
Liquid |
浓度 |
0.2 mg/mL |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2, with BSA |
内含物 |
0.09% sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2688084 |
Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.
Applications Reported: This MIH5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4 degrees C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
蛋白别名: B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1
基因别名: A530045L16Rik; B7h1; Cd274; Pdcd1l1; Pdcd1lg1; Pdl1
UniProt ID: (Mouse) Q9EP73
Entrez Gene ID: (Mouse) 60533
分子生物学功能:
immunoglobulin receptor superfamily
