Invitrogen

CD64 Monoclonal Antibody (X54-5/7.1), APC, eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1个已发表图片

4 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 3

CD64 Antibody (17-0641-82) in Flow

BALB/c mouse bone marrow cells were Fc blocked with CD16/CD32 Antibody (Product # 14-0161-82) and normal mouse serum, and stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and 0.25 µg of Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (left) or 0.25 µg of CD64 Monoclonal Antibody, APC (right). All cells were used for analysis.

Product Image Gallery

CD64 Antibody — Staining of mouse bone marrow. As expected based on the known relative expression patterns, CD64 clone X54-5/7.1 stains monocytes with the highest intensity (purple histogram) and B cell precursors with lower intensity (orange and blue histograms). Details: Mouse bone marrow was Fc blocked, stained with CD64 (clone X54-5/7.1), and co-stained with CD45R (B220) (clone RA3-6B2) and CD43 (clone eBioR2/60). Cells in the indicated color gates were used for analysis. {RE}

产品信息

17-0641-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

X54-5/7.1

抗原

BALB/c mouse CD64-human IgG Fc fusion protein

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2735010

产品详细信息

Description: This X54-5/7.1 monoclonal antibody recognizes allelic variants a and b of mouse CD64, a high affinity Fc binding receptor also known as Fc-gammaR1 or Fcgr1. Alloform a is present in the following mouse strains: C57BL/6, BALB/c, DBA/2, and alloform b in strains: C3H/HeJ, CBA/J, NZW, SJL/J, 129/SvJ. In addition this antibody has been reported positive in strains: AKR, ALR, BUB, C58, CE, HRS,MRL, MON, NZB, NZO, NZW, PL, SJL, ST and SWR. This clone X54-5/7.1 will not recognize the allelic variant d, present in NOD/Lt mice. This antibody has been reported to stain RAW264.7 cell line.

Applications Reported: This X54-5/7.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This X54-5/7.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

靶标信息

CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice.

Science advances

Liu J,Lane S,Lall R,Russo M,Farrell L,Debreli Coskun M,Curtin C,Araujo-Gutierrez R,Scherrer-Crosbie M,Trachtenberg BH,Kim J,Tolosano E,Ghigo A,Gerszten RE,Asnani A

Published figure using CD64 monoclonal antibody (Product # 17-0641-82) in Flow Cytometry

Fri Dec 23 00:00:00 EST 2022

Protective role of cortistatin in pulmonary inflammation and fibrosis.

British journal of pharmacology

Barriga M,Benitez R,Ferraz-de-Paula V,Garcia-Frutos M,Caro M,Robledo G,O'Valle F,Campos-Salinas J,Delgado M

17-0641-82 was used in Flow Cytometry to conclude that cortistatin-based therapies could emerge as attractive candidates to treat severe ALI/ARDS, including SARS-CoV-2-associated ARDS.

Mon Nov 01 00:00:00 EDT 2021

The Cytokine TGF-β Induces Interleukin-31 Expression from Dermal Dendritic Cells to Activate Sensory Neurons and Stimulate Wound Itching.

Immunity

Xu J,Zanvit P,Hu L,Tseng PY,Liu N,Wang F,Liu O,Zhang D,Jin W,Guo N,Han Y,Yin J,Cain A,Hoon MA,Wang S,Chen W

17-0641 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying itching associated with cutaneous wound healing, focusing on the contribution of soluble factors released during healing.

Tue Aug 18 00:00:00 EDT 2020

The cis-Regulatory Atlas of the Mouse Immune System.

Cell

Yoshida H,Lareau CA,Ramirez RN,Rose SA,Maier B,Wroblewska A,Desland F,Chudnovskiy A,Mortha A,Dominguez C,Tellier J,Kim E,Dwyer D,Shinton S,Nabekura T,Qi Y,Yu B,Robinette M,Kim KW,Wagers A,Rhoads A,Nutt SL,Brown BD,Mostafavi S,Buenrostro JD,Benoist C

17-0641-82 was used in Flow Cytometry to generate matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascade.

Thu Feb 07 00:00:00 EST 2019

生物信息学

蛋白别名: CD64; CD64 antigen; Fc-gamma receptor 1; Fc-gamma RI; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; IgG high affinity Fc receptor; RP11-196G18.2

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基因别名: AI323638; AV092959; CD64; Fcg1; FcgammaRI; Fcgr1; IGGHAFC

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UniProt ID: (Mouse) P26151

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Entrez Gene ID: (Mouse) 14129

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功能

IgG receptor activity IgG binding leukotriene receptor binding immunoglobulin receptor superfamily

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