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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Desmin Antibodies

          Invitrogen

          Desmin Monoclonal Antibody (DE-U-10), eBioscience™

          高级验证
          2个已发表图片
          1 篇参考文献
          查看其他36个Desmin抗体

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          说明书
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          技术支持
          说明书
          实验方案
          问题与答案
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          Cite Desmin Monoclonal Antibody (DE-U-10), eBioscience™

          • 抗体检测数据 (10)
          • 已发表图片 (2)
          • 高级验证 (5)
          Desmin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Desmin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 17

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          Desmin Antibody (14-9747-82) in ICC/IF

          Immunofluorescence analysis Desmin was performed using 70% confluent log phase RD cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Desmin Monoclonal Antibody (DE-U-10), eBioscience™ (Product 14-9747-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175)... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          Desmin Antibody in Immunocytochemistry (ICC/IF)
          Desmin Antibody in Immunocytochemistry (ICC/IF)
          Desmin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Desmin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Desmin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Desmin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Desmin Antibody in Western Blot (WB)
          Desmin Antibody in Western Blot (WB)
          Desmin Antibody in Western Blot (WB)
          Desmin Antibody in Western Blot (WB)
          Desmin Antibody in Immunohistochemistry (IHC)
          Desmin Antibody in Immunohistochemistry (IHC)
          Desmin Antibody
          Desmin Antibody
          Desmin Antibody
          Desmin Antibody
          Desmin Antibody

          产品信息

          14-9747-82

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          5 µg/mL
          -

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫组化(石蜡) (IHC (P))

          5 µg/mL
          -

          免疫细胞化学 (ICC/IF)

          5 µg/mL
          -
          产品规格

          种属反应

          Bovine, Chicken, Cat, Goat, Hamster, Human, Mouse, Sheep, Pig, Rabbit, Rat

          宿主/亚型

          Mouse / IgG1, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          DE-U-10

          偶联物

          Unconjugated Unconjugated Unconjugated
          • eFluor 660
          • Request custom conjugation

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.09% sodium azide

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2572976

          产品详细信息

          Description: The monoclonal antibody DE-U-10 recognizes desmin. Desmin is the protein subunit of class-III intermediate filaments. Desmin is found predominantly in skeletal, cardiac, and smooth muscle. Desmin plays a role in muscle cell development and differentiation, muscle cell architecture and structure, and mitochondrial function. Desmin forms scaffolds around the Z-disk of sarcomeres in muscle cells. Desmin knockout mice exhibit defects in skeletal, smooth, and cardiac muscle and have impaired mitochondrial function. Mutations in Desmin result in conditions such as desmin-related myopathy (DRM), cardiomyopathy dilated type 1I (CMD1I), and neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome).

          The DE-U-10 antibody recognizes human, mouse, rat, feline, bovine, goat, hamster, sheep, porcine, rabbit, and chicken desmin. The DE-U-10 antibody will also label tumors derived from muscle tissue, such as leiomyomas and rhabdomyosarcomas.

          Applications Reported: This DE-U-10 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

          Applications Tested: This DE-U-10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This DE-U-10 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized mouse cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          靶标信息

          Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: CMD1I; Desmin; desmin-like protein; FLJ12025; FLJ39719; FLJ41013; FLJ41793; intermediate filament protein; MFM1; muscle-specific intermediate filament desmin; muscle-specific intermediate filament protein; mutant desmin p.K241E; SCPNK

          查看更多 收起

          基因别名: CSM1; CSM2; DES; I79_018932; LGMD2R

          查看更多 收起

          UniProt ID: (Bovine) O62654, (Pig) P02540, (Human) P17661, (Rat) P48675, (Mouse) P31001

          查看更多 收起

          Entrez Gene ID: (Sheep) 101117308, (Bovine) 280765, (Pig) 396725, (Rabbit) 100328890, (Goat) 102180712, (Chicken) 395906, (Human) 1674, (Rat) 64362, (Mouse) 13346, (Chinese hamster) 100764990

          查看更多 收起

          功能
          cytoskeletal protein binding identical protein binding protein binding structural constituent of cytoskeleton structural molecule activity
          参与通路
          intermediate filament organization muscle organ development intermediate filament polymerization muscle contraction cytoskeleton organization regulation of heart contraction muscle filament sliding
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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