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Invitrogen
E4BP4 (NFIL3) Monoclonal Antibody (MABA223), Alexa Fluor™ 647, eBioscience™
FIGURE: 1 / 3
E4BP4 (NFIL3) Antibody (51-9812-42) in Flow
Normal human peripheral blood cells were stimulated overnight with CD3 and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0037-85 and Product # 16-0289-85). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD20 Monoclonal Antibody, FITC (Product # 11-0209-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 647 (Product # 51-4714-81) (left) or E4BP4 (NFIL3) Monoclonal Antibody, Alexa Fluor 647 (right). Total viable cells were used for an... View More
Product Details
51-9812-42
Applications
Tested Dilution
Publications
产品规格
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
分类
Monoclonal
类型
Antibody
克隆号
MABA223
Immunogen
21H2-hNFIL3 (aa 163-462)
Conjugate
Alexa Fluor™ 647
Excitation/Emission Max
650/671 nm
View spectra
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
RRID
AB_2811849
Product Specific Information
Description: This MABA223 monoclonal antibody recognizes human E4BP4 (NFIL3), a basic leucine zipper transcription factor first identified for its binding to the promoters of the adenovirus E4 protein and human IL-3. E4BP4 is involved in the transcriptional control of the mammalian circadian clock, motor neuron growth and survival, regulation of von Willbrand factor expression, and osteoblast function. In addition, it is required for the development of NK and NKT cells as well as CD8a+ dendritic cells. Although relatively high levels of mRNA are reported in resting NK and NKT cells, studies at eBioscience have observed expression of E4BP4 protein in lymphocytes only after activation.
Applications Reported: This MABA223 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MABA223 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
Target Information
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Flow Cytometry
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Bioinformatics
Protein Aliases: E4 promoter-binding protein 4; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NF IL3A; nuclear factor interleukin 3 regulated protein; Nuclear factor interleukin-3-regulated protein; OTTHUMP00000021633; Transcriptional activator NF-IL3A
Gene Aliases: E4BP4; IL3BP1; NF-IL3A; NFIL3; NFIL3A
UniProt ID: (Human) Q16649
Entrez Gene ID: (Human) 4783
Molecular Function:
basic leucine zipper transcription factor
DNA-binding transcription factor
gene-specific transcriptional regulator
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
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