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Invitrogen
FAM105B Polyclonal Antibody
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FAM105B Antibody (PA5-143981) in ICC/IF
Immunocytochemistry analysis of FAM105B in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in FAM105B Polyclonal antibody (Product # PA5-143981) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropria... View More
产品信息
PA5-143981
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human OTULIN recombinant protein (Position: A49-L352).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.01mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HELA whole cell, human THP-1 whole cell, human K562 whole cell, rat liver tissue, mouse liver tissue. IHC: human appendicitis tissue, human bladder cancer tissue, human tonsil tissue, human placenta tissue. ICC/IF: SIHA cell. Flow: 293T cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Otulin is a deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Deubiquitinating enzyme otulin; family with sequence similarity 105, member B; gumby; OTU deubiquitinase with linear linkage specificity; OTU domain-containing deubiquitinase with linear linkage specificity; protein FAM105B; Ubiquitin thioesterase Gumby; Ubiquitin thioesterase otulin
基因别名: C79097; FAM105B; GUM; m3Sapc; m7-1Sapc; OTULIN
UniProt ID: (Human) Q96BN8, (Mouse) Q3UCV8
Entrez Gene ID: (Human) 90268, (Mouse) 432940, (Rat) 100362554
angiogenesis
sprouting angiogenesis
immune system process
proteolysis
Wnt signaling pathway
negative regulation of NF-kappaB transcription factor activity
innate immune response
negative regulation of inflammatory response
canonical Wnt signaling pathway
nucleotide-binding oligomerization domain containing 2 signaling pathway
protein linear deubiquitination
regulation of tumor necrosis factor-mediated signaling pathway
