Proteintech

HDAC8 Polyclonal Antibody

Name: HDAC8 Polyclonal Antibody
Brand: Proteintech
SKU: 17548-1-AP

19 篇参考文献

查看其他19个HDAC8抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)

HDAC8 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

HDAC8 Antibody (17548-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 17548-1-AP (HDAC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

HDAC8 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Immunogen image

Immunogen Image HDAC8 Polyclonal Antibody

产品信息

17548-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看18篇已发表文章 18篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:500

免疫沉淀 (IP)

0.5-4.0 µg

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

HDAC8 Fusion Protein Ag11696 (1-377 aa encoded by BC050433)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.14 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.

Immunogen sequence: MEEPEEPAD SGQSLVPVYI YSPEYVSMCD SPAKIPKRAS MVHSLIEAYA LHKQMRIVKP KVASMEEMAT FHTDAYLQHL QKVSQEGDDD HPDSIEYGLG YDCPATEGIF DYAAAIGGAT ITAAQCLIDG MCKVAINWSG GWHHAKKDEA SGFCYLNDAV LGILRLRRKF ERILYVDLDL HHGDGVEDAF SFTSKVMTVS LHKFSPGFFP GTGDVSDVGL GKGRYYSVNV PIQDGIQDEK YYQICESVLK EVYQAFNPKA VVLQLGADTI AGDPMCSFNM TPVGIGKCLK YILQWQLATL ILGGGGYNLA NTARCWTYLT GVILGKTLSS EIPDHEFFTA YGPDYVLEIT PSCRPDRNEP HRIQQILNYI KGNLKHVV (1-377 aa encoded by BC050433)

靶标信息

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。