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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • IL-13 Antibodies

          Invitrogen

          IL-13 Monoclonal Antibody (eBio13A), PE-Cyanine7, eBioscience™

          高级验证
          41个已发表图片
          47 篇参考文献
          查看其他65个IL-13抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite IL-13 Monoclonal Antibody (eBio13A), PE-Cyanine7, eBioscience™

          • 抗体检测数据 (1)
          • 已发表图片 (41)
          • 高级验证 (1)
          IL-13 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          IL-13 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          图: 1 / 43

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          IL-13 Antibody (25-7133-82) in Flow

          CD4+ BALB/c splenocytes were stimulated for 10-days under Th2 polarizing conditions. Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) for 5 hours. Following restimulation, cells were fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and then stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82) and 0.25 µg of Rat IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4301-82) (left) or 0.25 µg of Anti-Mouse I... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          IL-13 Antibody in Flow Cytometry, ELISA (Flow, ELISA)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody in Flow Cytometry (Flow)
          IL-13 Antibody

          产品信息

          25-7133-82

          应用
          建议稀释比
          已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          流式细胞分析 (Flow)

          0.5 µg/test
          查看45篇已发表文章 45篇已发表文章

          酶联免疫吸附实验 (ELISA)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Mouse

          已发表种属

          Human, Mouse

          宿主/亚型

          Rat / IgG1, kappa

          推荐的同型对照

          Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          eBio13A

          偶联物

          PE-Cyanine7 PE-Cyanine7 PE-Cyanine7
          • Unconjugated
          • Alexa Fluor 488 
          • APC-eFluor 780
          • Brilliant UV 737
          • Brilliant UV 805
          • eFluor 450
          • eFluor 660
          • Functional Grade
          • PE 
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Request custom conjugation

          激发/发射光谱

          569/780 nm 查看光谱 spectra

          形式

          Liquid

          浓度

          0.2 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.09% sodium azide

          保存条件

          4°C, store in dark, DO NOT FREEZE!

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2573530

          产品详细信息

          Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

          Applications Reported: This eBio13A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

          Applications Tested: This eBio13A antibody has been tested by intracellular staining of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          靶标信息

          IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          生物信息学

          蛋白别名: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

          查看更多 收起

          基因别名: Il-13; Il13

          查看更多 收起

          UniProt ID: (Mouse) P20109

          查看更多 收起

          Entrez Gene ID: (Mouse) 16163

          查看更多 收起

          功能
          cytokine activity cytokine receptor binding interleukin-13 receptor binding
          参与通路
          microglial cell activation positive regulation of immunoglobulin production inflammatory response immune response regulation of proton transport positive regulation of B cell proliferation positive regulation of connective tissue growth factor production negative regulation of NAD(P)H oxidase activity response to nicotine positive regulation of tyrosine phosphorylation of Stat6 protein positive regulation of macrophage activation positive regulation of ion transport positive regulation of mast cell degranulation positive regulation of smooth muscle cell proliferation positive regulation of protein secretion positive regulation of release of sequestered calcium ion into cytosol cellular response to cytokine stimulus negative regulation of transforming growth factor beta production negative regulation of neuron death negative regulation of complement-dependent cytotoxicity positive regulation of pancreatic stellate cell proliferation negative regulation of endothelial cell apoptotic process
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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