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Invitrogen
IRF8 Recombinant Mouse Monoclonal Antibody (GW4CML3)
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IRF8 Antibody (740242M) in WB
Western blot was performed using IRF8 Recombinant Mouse Monoclonal Antibody (GW4CML3) (Product # 740242M) and ~50 kDa band corresponding to IRF8 was observed in THP-1, Raji, Ramos as compared to K-562, T98-G which are reported to be low for IRF8 expression. Whole cell extracts (30 µg lysate) of THP1 (Lane 1), Raji (Lane 2), Ramos (Lane 3), K-562 (Lane 4) and T98-G (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0326BOX). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot™ 3 W... View More
产品信息
740242M
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
GW4CML3
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
产品详细信息
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: H-ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8; unnamed protein product
基因别名: H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8
UniProt ID: (Human) Q02556
Entrez Gene ID: (Human) 3394
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
plasmacytoid dendritic cell differentiation
germinal center B cell differentiation
follicular B cell differentiation
immune system process
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
phagocytosis
autophagy
immune response
response to bacterium
myeloid cell differentiation
regulation of type I interferon production
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
defense response to bacterium
defense response to protozoan
positive regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to lipopolysaccharide
cellular response to interferon-gamma
dendritic cell differentiation
defense response to other organism
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