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Invitrogen
Metallothionein Monoclonal Antibody (2B5), FITC
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Metallothionein Antibody (MA5-45899) in WB
Western Blot analysis of Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged showing detection of 36 kDa Metallothionein protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged. Load: 1 µg. Blocking: 5% Skim Milk powder in TBST. Samples were incubated with Metallothionein monoclonal antibody (Product # MA5-45899) at 1:1,000 for 2 hours at RT, followed by Goat Anti-Mouse IgG:HRP at 1:5,000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Pre... View More
产品信息
MA5-45899
产品规格
种属反应
Bacteria
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2B5
抗原
Recombinant full length Pseudomonas aeruginosa Metallothionein protein
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
内含物
no preservative
保存条件
4°C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
A 1:1,000 dilution of MA5-45899 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein.
靶标信息
Metallothioneins (MTs) are a family of low molecular weight, heavy metal-binding proteins characterized by a high cysteine content and lack of aromatic amino acids. MTs bind 7 to 12 heavy metal atoms per molecule of protein. They are ubiquitous in the animal and plant kingdoms and are also found in prokaryotes. In mammals, the cysteine residues are absolutely conserved and serve to coordinate heavy metal atoms such as zinc, cadmium, and copper via mercaptide linkages.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CES 1; CES1; Metallothionein; Metallothionein 2; Metallothionein 2A; Metallothionein IA; Metallothionein II; Metallothionein2; MGC32848; MT 1A; MT 2; MT 2A; MT IA; MT II; MT2; MT2A; Probable pmtA
