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Phospho-FOXO4 (Thr28) Antibody (710880) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized A431 cells for detection of FoxO4 (pT28) using Anti-FoxO4 (pT28) Recombinant Rabbit Polyclonal Antibody (Product # 710880, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection ... View More
产品信息
710880
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
12HCLC
抗原
phosphopeptide corresponding to human FOXO4 (aa 25-33)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532776
产品详细信息
This antibody is predicted to react with Monkey, Goat, Pig, Bovine, Dog, Rat and Mouse.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
FOXO4 (Forkhead box O4) is encoded by the FOXO4 gene located on the X chromosome. It is also known as AFX1 or MLLT7 (Myeloid/Lymphoid or Mixed-Lineage Leukemia (Trithorax Homolog, Translocated To, 7) and is a member of the O class of forkhead/ winged helix family of transcription factors along with FOXO1, FOXO3 and FOXO6. FOXO4 is abundantly expressed in skeletal muscle and adipose tissue. FOXO4 binds to the Insulin Responsive Element (IRE) and activates downstream targets of Insulin/IGF1 signaling such as IGFBP1. However, the activation of another downstream target of the Insulin/IGF1 pathway, Akt, leads to phosphorylation of FOXO4 on specific Ser/Thr residues. This phosphorylation mediates binding of FOXO4 to 14-3-3 and eventual exit of FOXO4 from the nucleus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AFXzeta; Fork head domain transcription factor AFX1; Forkhead box protein O4; FOXO4b; MGC120490; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7; OTTHUMP00000023497
基因别名: AFX; AFX1; FOXO4; MLLT7
UniProt ID: (Human) P98177
Entrez Gene ID: (Human) 4303
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell cycle arrest
mitotic G2 DNA damage checkpoint
muscle organ development
negative regulation of cell proliferation
insulin receptor signaling pathway
negative regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
negative regulation of smooth muscle cell differentiation
negative regulation of G0 to G1 transition
positive regulation of cell cycle arrest
