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Phospho-ULK1 (Ser758) Antibody (PA5-78251) in WB
Western Blot using Phospho-ULK1 (Ser758) Polyclonal Antibody (Product # PA5-78251). Untreated (–) and treated (+) MCF7 whole cell extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Phospho-ULK1 (Ser758) Polyclonal Antibody (Product # PA5-78251) diluted at 1:500.
Product Details
PA5-78251
产品规格
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ULK1 (phospho Ser758). The exact sequence is proprietary.
Conjugate
Unconjugated
形式
Liquid
浓度
0.3 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2736429
Product Specific Information
Positive Control: MCF7, MCF-7 (starvation for 16 hr, incubated in EBSS for 4 hr, and 200 nM Rapamycin treatment for 0.5 hr)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; ULK 1; UNC 51; Unc-51-like kinase 1
Gene Aliases: ATG1; ATG1A; hATG1; KIAA0722; ULK1; UNC51; Unc51.1
UniProt ID: (Human) O75385
Entrez Gene ID: (Human) 8408
Molecular Function:
non-receptor serine/threonine protein kinase
protein phosphorylation
signal transduction
protein localization
regulation of autophagy
positive regulation of autophagy
macroautophagy
positive regulation of macroautophagy
peptidyl-serine phosphorylation
neuron projection regeneration
neuron projection development
negative regulation of protein complex assembly
cellular response to nutrient levels
response to starvation
protein autophosphorylation
axon extension
Performance Guarantee
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