FIGURE: 1 / 1
Positive Control: MCF7, MCF-7 (starvation for 16 hr, incubated in EBSS for 4 hr, and 200 nM Rapamycin treatment for 0.5 hr)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
Protein Aliases: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:22.214.171.124; FLJ38455; Serine/threonine-protein kinase ULK1; ULK 1; UNC 51; Unc-51-like kinase 1
Gene Aliases: ATG1; ATG1A; hATG1; KIAA0722; ULK1; UNC51; Unc51.1
UniProt ID: (Human) O75385
Entrez Gene ID: (Human) 8408
Molecular Function: non-receptor serine/threonine protein kinase