Invitrogen

RAP1GAP Polyclonal Antibody

Name: RAP1GAP Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-106715
Availability: InStock

查看其他14个RAP1GAP抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (5)

RAP1GAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 5

RAP1GAP Antibody (PA5-106715) in IHC (P)

Immunohistochemistry analysis of RAP1GAP in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RAP1GAP polyclonal antibody (Product # PA5-106715) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image RAP1GAP Polyclonal Antibody

产品信息

PA5-106715

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RAP1GAP(Accession P47736), corresponding to amino acid residues S484-S534.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2854383

产品详细信息

Antibody detects endogenous levels of total RAP1GAP.

靶标信息

Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.

仅用于科研。不用于诊断过程。未经明确授权不得转售。