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Invitrogen
UBC13 Polyclonal Antibody
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UBC13 Antibody (PA5-17010) in WB
Western blot was performed using anti-UBC13 Polyclonal Antibody (Product # PA5-17010) and a 17 kDa band corresponding to UBE2N was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HepG2 (Lane 1), HeLa (Lane 2), NIH:OVCAR-3 (Lane 3), BeWo (Lane 4), HL60 (Lane 5), MOLT4 (Lane 6), tissue extracts of Mouse Testis (Lane 7), Mouse Brain (Lane 8), Mouse Liver (Lane 9) and Mouse Ovary (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were the... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-17010
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues surrounding Ile10 of human Ubc13 protein
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
5 µg/mL
规格
0.5 µg
纯化类型
Antigen affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway. (Uniprot)
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: bendless protein; Bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; MGC131857; MGC8489; Ubc13; UbcH13; Ubiquitin carrier protein N; Ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N
基因别名: 1500026J17Rik; AL022654; BB101821; BLU; UBC13; UBE2N
UniProt ID: (Rat) Q9EQX9, (Mouse) P61089
Entrez Gene ID: (Rat) 116725, (Mouse) 93765
ubiquitin-protein transferase activity
ATP binding
ligase activity
ubiquitin-like protein transferase activity
ubiquitin protein ligase binding
ubiquitin binding
poly(A) RNA binding
ubiquitin protein ligase activity
ubiquitin conjugating enzyme activity
nucleotide binding
protein binding
ubiquitin-protein ligase
double-strand break repair via homologous recombination
DNA double-strand break processing
postreplication repair
ubiquitin-dependent protein catabolic process
protein ubiquitination
histone ubiquitination
positive regulation of histone modification
regulation of histone ubiquitination
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of DNA repair
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
positive regulation of ubiquitin-protein transferase activity
protein K63-linked ubiquitination
DNA repair
cellular response to DNA damage stimulus
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