Invitrogen

USP9X Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. 查看细节

查看其他11个USP9X抗体

说明书

Protocols

技术支持

说明书

Protocols

技术支持

Product Image Gallery

USP9X Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Probable ubiquitin carboxyl-terminal hydrolase FAF-X was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with USP9X Polyclonal Antibody (Product # PA5-23035) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A27034), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification.

USP9X Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of USP9X using a polyclonal antibody (Product # PA5-23035).

USP9X Antibody in Western Blot (WB) — Western Blot was performed using Anti-USP9X Polyclonal Antibody (Product # PA5-23035) and a 260 kDa band corresponding to Probable ubiquitin carboxyl-terminal hydrolase FAF-X was observed across across the cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), PC-3 (Lane 4) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

USP9X Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with Probable ubiquitin carboxyl-terminal hydrolase FAF-X siRNA and decrease in signal intensity was observed in Western Blot application using Anti-USP9X Polyclonal Antibody (Product # PA5-23035). {KD}

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:50

免疫细胞化学 (ICC/IF)

1:25-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Genomic peptide made to an internal region of the human Usp9X protein (within residues 1150-1300).

偶联物

Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 0.1% BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_11156993

产品详细信息

The target sequence has 95% sequence homology with rat and 80% sequence homology with zebrafish.

靶标信息

USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. As a deubiquitinating enzyme, USP9x has a critical function in tight junction biogenesis by controlling the levels of the exchange factor for Arf6 (EFA6), a protein shown to facilitate tight junction formation during a narrow temporal window preceding the establishment of cell polarity. USP9X is also located on the X-chromosome but escapes X-inactivation, and defects in USP9X may cause gonadal degeneration in Turner syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Deubiquitinating enzyme FAF-X; Drosophila fat facets related, X-linked; Fat facets homolog; Fat facets in mammals; fat facets protein related, X-linked; Fat facets protein-related, X-linked; fats facets protein related, X; hFAM; Probable ubiquitin carboxyl-terminal hydrolase FAF-X; Ubiquitin carboxyl-terminal hydrolase FAM; ubiquitin specific peptidase 9, X chromosome; ubiquitin specific protease 9 SSSRF- isoform; ubiquitin specific protease 9, X chromosome; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); Ubiquitin thioesterase FAF-X; ubiquitin thiolesterase FAF-X; ubiquitin-specific processing protease FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X

基因别名: 5730589N07Rik; AA407302; AA407699; AL022658; AL022749; DFFRX; FAF; FAF-X; Fafl; FAM; MRX99; MRXS99F; RGD1560056; USP9; USP9X

UniProt ID: (Human) Q93008, (Mouse) P70398

Entrez Gene ID: (Human) 8239, (Mouse) 22284, (Rat) 363445

分子生物学功能: cysteine protease