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Invitrogen
VAMP1 Polyclonal Antibody
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VAMP1 Antibody (PA1-765) in ICC/IF
Immunofluorescence analysis of VAMP1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with VAMP1 Rabbit Polyclonal Antibody (Product # PA1-765) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
产品信息
PA1-765
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues M(1) S A P A Q P P A E G T E G A A P G(18) C of rat VAMP-1.
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
PA1-765 detects vesicle associated membrane protein 1 (VAMP-1) from human, canine, mouse and rat tissues.
PA1-765 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 15 kDa protein representing VAMP-1 from rat brain whole protein extract.
The PA1-765 immunogen is a synthetic peptide corresponding to residues M(1) S A P A Q P P A E G T E G A A P G(18) C of rat VAMP-2. This immunizing peptide (Cat. # PEP-099) is available for use in neutralization and control experiments.
靶标信息
Synapotobrevins, syntaxins, and the synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Mutations in this gene are associated with autosomal dominant spastic ataxia 1. Multiple alternative splice variants have been described, but the full-length nature of some variants has not been defined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
