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Invitrogen
cGAS Polyclonal Antibody
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图: 1 / 9
cGAS Antibody (PA5-141097) in ICC/IF
Immunocytochemistry analysis of cGAS in SK-OV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Samples were incubated in cGAS Polyclonal antibody (Product # PA5-141097) using a dilution of 1:100 for 1 hour at room temperature, washed with PBS followed by Alexa Fluor®488 Goat anti-Rabbit IgG secondary antibody at a dilution of 1:1,000. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-141097
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Pig,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide within human cGAS aa 71-120.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.98 mg/mL
规格
198 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
cGAS (Cyclic GMP-AMP synthase) is a nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of 2', 5' phosphodiester linkage at the GpA step and the formation of 3', 5' phosphodiester linkage at the ApG step. This produces c[G(2',5')pA(3'5')p] responses. cGAS also binds cytosolic DNA directly, leading to the activation and synthesis of cGAMP. Then, a second messenger binds to and activates TMEM173/STING thereby triggering type-I interferon production. cGAS is also involved in innate immune sensorship of infection, detection of DNA from bacteria, response to cellular stress, DNA damage, and regulation of cellular senescence.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 2'3'-cGAMP synthase; C6orf150; C6orf150; cGAS is a cytosolic DNA sensor that induces interferons by producing the second messenger cGAMP; cGAMP synthase; Cyclic GMP-AMP synthase; DANGER family member 4; E330016A19; cGAS is a cytosolic DNA sensor that induces interferons by producing the second messenger cGAMP; h-cGAS; Mab-21 domain containing 1; Mab-21 domain-containing protein 1; MB21D1; protein MB21D1; unnamed protein product
基因别名: C6orf150; CGAS; D4; E330016A19Rik; h-cGAS; MB21D1
UniProt ID: (Pig) I3LM39
Entrez Gene ID: (Pig) 100516408, (Human) 115004, (Rat) 682147, (Mouse) 214763
DNA binding
chromatin binding
double-stranded DNA binding
ATP binding
GTP binding
phosphatidylinositol-4,5-bisphosphate binding
metal ion binding
cyclic-GMP-AMP synthase activity
protein binding
nucleosome binding
identical protein binding
protein homodimerization activity
molecular condensate scaffold activity
poly-ADP-D-ribose modification-dependent protein binding
activation of innate immune response
pattern recognition receptor signaling pathway
positive regulation of defense response to virus by host
regulation of immunoglobulin production
cytoplasmic pattern recognition receptor signaling pathway
cellular response to DNA damage stimulus
determination of adult lifespan
regulation of type I interferon production
positive regulation of type I interferon production
paracrine signaling
negative regulation of DNA repair
regulation of immune response
regulation of T cell activation
defense response to virus
cellular response to exogenous dsRNA
antiviral innate immune response
cGAS/STING signaling pathway
negative regulation of double-strand break repair via homologous recombination
positive regulation of cellular senescence
DNA repair
positive regulation of cAMP-mediated signaling
innate immune response
negative regulation of cGAS/STING signaling pathway
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