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Rise to the multiomics challenges of today and tomorrow
Be prepared for the world ahead with our most flexible instrument. Explore new capabilities in proteomics, metabolomics, lipidomics and glycomics without compromising ease-of-use. Enjoy the flexibility to support all quantitative proteomic workflows.
The Thermo Scientific Orbitrap Tribrid Apex MultiOmics Mass Spectrometer unifies deep discovery and confident quantitation on a single platform. From proteomics and metabolomics to lipidomics and glycomics, it delivers greater depth, speed, and confidence through enhanced ion parallelization, IR-assisted fragmentation, and flexible MSⁿ workflows.
TMTpro workflows, which include the Orbitrap Tribrid Apex MultiOmics MS, deliver high-performance sample multiplexing. New, exclusive targeted TMT MS³ acquisitions help increase throughput while capturing lower-abundance protein targets using adaptive real-time library searching. The first commercial IR-based laser further expands performance by enabling more efficient reporter ion release and improved characterization of complex post-translational modifications, including glycosylation.
From discovery to validation, the Orbitrap Tribrid Apex MultiOmics MS provides the flexibility and performance that researchers need to answer complex biological questions with confidence.
Applications
Power every omics workflow with unmatched performance and confidence.
Deeper proteome coverage
The Orbitrap Tribrid Apex MultiOmics MS enables deeper proteome coverage through improved scan efficiency and ion utilization. Enhanced ion parallelization and scan speeds up to 75 Hz for DIA and DDA MS² experiments increase peptide and protein identifications while maintaining throughput.
Identification gains observed with Orbitrap Tribrid Apex MultiOmics MS compared with Thermo Scientific Orbitrap Ascend MultiOmics Tribrid MS using the same sample and method. The Orbitrap Tribrid Apex MultiOmics MS increased detected precursors, peptides, and protein groups, with peptide identifications increasing from 17,225 to 44,802.
Improved multiplexed protein quantitation
New IR-assisted fragmentation enhances reporter ion generation in TMT multiplexing experiments, increasing reporter ion signal-to-noise for more precise and sensitive quantitation, including improved accuracy for low-abundance proteins. The Orbitrap Tribrid Apex MultiOmics MS also enables smart targeted TMT workflows using adaptive real-time library searching (aRTLS) to support validation experiments.
On the left is the comparison of TMT reporter ion S/N from SPS MS³ using HCD or IRMPD fragmentation, and on the right figure is the quantified peptides from a TMTpro 18-plex human cell line discovery experiment.
Smart targeted TMT method using aRTLS of 138 low abundant E. Coli peptides in yeast proteome increases identifications compared to TMT with real-time search alone. Multiplexed analysis allows for 35 samples in one injection.
What scientists are saying about Orbitrap Tribrid Apex MultiOmics MS for TMT multiplexing experiments
"The Orbitrap Tribrid Apex MultiOmics MS continues to set the standard for multiplexed quantitative proteomics. The addition of the new IR laser significantly enhances reporter ion signal intensity in TMT experiments, improving both sensitivity and quantitative precision—particularly for low-abundance proteins. Combined with SPS MS3 capabilities, these advances strengthen interference control while preserving depth of coverage. This translates directly into more confident, reproducible quantification across large-scale proteomic studies."
Prof. Steven Gygi, Ph.D., Harvard Medical School
Confident glycopeptide characterization
For glycoproteomics, IR-assisted ETD improves fragmentation efficiency, increasing glycopeptide sequence coverage and supporting more accurate glycosylation site localization to help resolve complex post-translational modification (PTM) heterogeneity with greater confidence.
PTMs are vital biological signals, but difficult to analyze. The improved parallelization and inclusion of the IR-laser makes digging deeper possible.
Nearly double unique O-linked glycopeptides identifications via improved parallelization and introduction of IR-laser activation prior to EThcD fragmentation.
What scientists are saying about Orbitrap Tribrid Apex MultiOmics MS for glycoproteomics
"Electron transfer dissociation continues to be highly advantageous for characterizing numerous post-translational modifications, especially glycosylation that is the focus of my research group.
The improvement made to ETD technology on Orbitrap Tribrid Apex MultiOmics MS, including significant speed increases for more high-quality spectra and new modalities for supplemental activation to improve the information available in by each spectrum, directly translate to better characterization of the glycoproteome for both routine and bespoke experiments."
Prof. Nicholas Riley, Ph.D., Assistant Professor of Chemistry, University of Washington
Full immunopeptide sequence verification
The Orbitrap Tribrid Apex MultiOmics MS uses EThcD to provide complementary b/y and c/z ion coverage, helping verify MHC-bound immunopeptides with greater sequence completeness. This is especially important for confirming anchor residues that influence HLA binding, immune recognition, and downstream immunotherapy or biomarker decisions.
Precision immunopeptidomics can transform discovery into high-confidence, actionable insights for immunotherapy and biomarker development. For this, accurate sequencing of MHC-bound immunopeptides is essential, as single anchor residues dictate binding specificity and immune recognition.
However, discovery alone is not enough—precision immunopeptidomics demands confident verification of every amino acid. Orbitrap Tribrid Apex MultiOmics MS with EThcD elevate immunopeptidomics by delivering comprehensive b/y and c/z ion coverage, ensuring full sequence confirmation—including critical anchor residues.
What scientists are saying about immunopeptidomics on Orbitrap Tribrid Apex MultiOmics MS
"In immunopeptidomics, every amino acid matters, and a single misassigned residue can alter therapeutic strategy. Orbitrap Tribrid Apex MultiOmics MS delivers increased ion coverage and exceptionally clean spectra, substantially strengthening analytical confidence in peptide–HLA identification. When antigen identification may guide treatment decisions, that level of confidence is not optional; it is essential."
Prof. Pouya Faridi, Ph.D., Monash University
Faster, smarter lipid identification
The Orbitrap Tribrid Apex MultiOmics MS delivers the sensitivity, resolution, and MS2 speed needed to confidently identify and quantify diverse lipid species in complex samples. High-resolution MS/MS helps distinguish lipid classes, structural features, and isobaric species across a wide dynamic range.
With Real-Time Library Searching (RTLS), lipid identifications can be made during acquisition, enabling adaptive data collection based on what is detected in real time. This helps increase scan efficiency, improve spectral quality, and accelerate confident lipidomics discovery.
Templated methods improve ease-of-use for complex experimental designs. RTLS allows for real-time classification of the lipids for specific analysis in a branched method.
Improved parallelization allows for more scans even with allowing for longer injection times. Translating to more identifications with higher quality MS2.
FAQs
The increased parallelization means that ions are moved through the different sections of the mass spectrometer more efficiently. In previous systems, ions often had to wait to be collected or transferred until another packet of ions had moved through the system. Now, ions are more efficiently packeted into each section of the mass spectrometer, allowing more time for ion accumulation while other processes are occurring. For example, while ions are reacting in the ion trap, the next packet can be collected more efficiently.
This translates to more accumulation time regardless of the Orbitrap resolution setting or scan type. However, the benefit is more pronounced in certain experiment types, such as those involving the ion trap, ETD, IR-laser fragmentation, or MS³. Overall, this provides more ion collection time per scan, which can boost signal-to-noise or enable more scans across the peak without sacrificing signal-to-noise compared to the previous parallelization setup. Either outcome leads to improved results for discovery or quantitation.
TMTpro provides many benefits on any Orbitrap-based mass spectrometer. Multiplexing helps reduce systematic errors in quantitation, improving accuracy while also allowing more samples to be analyzed in a single run, saving instrument time.
Specifically on the Orbitrap Tribrid Apex MS, we benefit from well-established methods using RTLS with Synchronous Precursor Selection (SPS) MS³ experiments. In this approach, specific fragment ions from the MS² spectrum of a precursor are selected after being identified as part of the peptide of interest via RTLS. Those fragments contain the reporter ions, so when they are fragmented for MS³, the reporter ions are much more likely to originate from the correct peptide.
This methodology, combined with increased scan speed through improved parallelization and the new IR laser for more efficient reporter ion fragmentation, results in significant boosts in TMT efficiency. We also introduce a smart targeted TMT methodology powered by adaptive RTLS (aRTLS), enabling discovery TMT to be coupled with specific targets. Learn more about TMT multiplexing technology.
aRTLS is a technology adapted from Professor Steven Gygi at Harvard Medical School’s GoDig method. This processing tool allows scientists using TMT methods to adapt their libraries to target specific classes of compounds, greatly increasing the odds that lower-abundance species within those classes are quantified with high-quality MS³ reporter ion spectra.
For Research Use Only. Not for use in diagnostic procedures.