Search
Rise to the small molecule challenges of today and tomorrow
For small molecule research, the Thermo Scientific Orbitrap Tribrid Apex Small Molecule Mass Spectrometer brings the latest advancements in Tribrid hardware onto a dedicated platform, along with optimal characterization ability. From discovery and structural characterization in the Orbitrap, sensitive quantitation in the linear ion trap, and all three at the same time using the Simultaneous Quantitation and Discovery (SQUAD) analysis workflow, the unique architecture and template methods make it all possible, with customer usability at the forefront.
Study the unknown, and perform advanced discovery methods with intelligent acquisition. For pharmaceutical research, metabolomics, lipidomics, and natural products, experience experimental flexibility and tried and true template methods to collect the data that answers your analytical questions.
Prepare for your future experimental needs with field upgradeable options and an upgrade path to the MultiOmics Edition.
What scientists are saying about Orbitrap Tribrid Apex Small Molecule MS
"Fungal biofilms promote chronic infections and antifungal resistance to severely limit treatment options against disease. With the Orbitrap Tribrid Apex Small Molecule mass spectrometer, instrument driven multi-stage and alternative fragmentation schemes are uncovering a hidden world of small molecules that transform natural products into precision antibiofilm therapeutics."
Jennifer Geddes-McAllister, Ph.D., Canada Research Chair/ Associate Professor, University of Guelph
Application capabilities
Increased number of scans
Improved parallelization, front-end optics, and faster polarity switching increase scan throughput, enabling higher-quality quantitation and better detection of low-abundance species.
Achieve faster polarity switching with more scans in the same amount of time. Resulting in more points across the peak for higher quality quantitation.
Hardware and software improvements lead to over 4 times more signal. With more ions in the same time frame, peak areas for pharmaceutical standards increased by 60-400%.
Achieve 5x lower quantification of nitrosamine standard compared to Orbitrap IQ-X Tribrid MS.
Simultaneous Quantitation and Discovery (SQUAD)
For fully parallelized acquisition of both targeted and untargeted data, Orbitrap Tribrid mass spectrometers utilize both Orbitrap and ion trap mass analyzers simultaneously. During SQUAD analysis on Orbitrap Tribrid Apex Small Molecule MS, full scan MS1 profiling is performed in the Orbitrap for untargeted discovery data acquisition while known metabolites of interest are detected using targeted-MS2 experiments with sensitive ion-trap detection. The improved ion-funnel and increased ion flux effect overall sensitivity when compared to previous generation small molecule Tribrids.
Compared to full scan Orbitrap quantitation, targeted MS2 ion-trap methods often exhibit an additional order of magnitude linearity at the low end of the curve achieving a linear dynamic range of seven orders of magnitude.
The increased sensitivity of Orbitrap Tribrid Apex Small Molecule MS provides up to 10x improvement over the previous small molecule generation Orbitrap Tribrid MS.
Reveal structural complexity with MSn and UVPD
Lipids have a high capacity for isomers due to the positions of their double bonds or even a simple change in position for the acyl chains. Elucidation of the isomeric species requires in-depth mass spectrometry. The ion trap allows for MSn experiments with alternate fragmentation, like UV-laser based dissociation. By utilizing pre-built templates and multiple tools available on the system, distinguishing small molecule isomers is made possible.
Two isomeric lipids differ only in the position of the acyl chains. UVPD MS3 produces distinct fragments that distinguish isomers for confident structural annotation.
FAQs
There are significant hardware and software changes between the previous generation Orbitrap Tribrid MS and the Orbitrap Tribrid Apex Small Molecule MS. Improvements include the addition of a brighter source with the letter-box-shaped ion transfer tube and a front ion routing multiple, which improves the parallelization of ion transfer and increases the number of scans possible in the same timeframe. IR-laser for alternate fragmentation capabilities has also been added.
The Small Molecule edition is the most unique edition within the Orbitrap Tribrid Apex MS line. It is tailored for the specific needs of the small molecule community without the extra capabilities needed for proteomics or protein structural analysis. This means that certain features, including TurboTMT, data-independent acquisition, and data-dependent acquisition for charge state above +2 are not available. The mass range is limited to lower m/z values. This allows researchers to focus on their application space without features they will not use. However, if you become interested in expanding your capabilities in the future, we offer field upgradeability to the MultiOmics Edition, to ensure your future needs are met.
The UV-laser option is recommended for the Orbitrap Tribrid Apex Small Molecule MS.
Yes, but the roughly 30% increase in MS1 fragment for Phenylalanine is also accompanied by a 10x improvement in sensitivity and another order of magnitude linear range due to the new letter-box opening ion transfer tube. The Orbitrap IQ-X Tribrid MS has the round bore opening ion transfer tube, which helps reduce source fragmentation, but also reduces ion transfer into the system.
For Research Use Only. Not for use in diagnostic procedures.