Bioanalysis Applications

Applications across therapeutic modalities

From small molecules to proteins and nucleic acids, our bioanalytical solutions are built to deliver accurate, efficient quantitation across a range of therapeutic modalities. Whether you're conducting exploratory research or supporting regulated studies, our technologies help you generate confident, high-quality data for a variety of bioanalytical applications, including small molecule bioanalysis, protein-based bioanalysis, and nucleic acid-based bioanalysis.

 

Explore each application area to see how our tools can support your workflow from discovery through development.

Small molecule bioanalysis

Small molecule bioanalysis is the precise chemical measurement of low molecular weight compounds, typically under 900 Daltons, within complex biological matrices like serum or plasma. These small molecules, which include many traditional pharmaceuticals, play a crucial role in regulating biological processes. Throughout the drug development lifecycle, from preclinical studies to clinical trials, accurate and reliable bioanalysis is essential for quantifying drug concentrations and metabolites in biological samples.

 

Liquid chromatography coupled with mass spectrometry has become the gold standard for small molecule bioanalysis, offering the sensitivity, selectivity, and reproducibility needed to meet stringent regulatory requirements and deliver confident results.

Sensitive, robust quantitative analysis of a mixture of drug candidates in plasma using a Thermo Scientific TSQ Altis Triple Quadrupole Mass Spectrometer

Accurate quantitation of drug candidates in plasma is vital for drug development. LC-MS delivers the sensitivity and reliability needed for confident results. Read this application note to discover how the Thermo Scientific TSQ Altis Triple Quadrupole Mass Spectrometer supports robust small molecule bioanalysis.

Protein-based bioanalysis

In the evolving landscape of pharmaceutical development, accurate and sensitive bioanalysis of therapeutic proteins is critical for ensuring safety, efficacy, and regulatory compliance. Whether you're quantifying biotherapeutics or monitoring biomarkers, the right analytical tools can make all the difference.​ Thermo Fisher Scientific offers a robust portfolio of chromatography and mass spectrometry platforms designed to meet the complex demands of protein-based bioanalysis.

A fast and simple immuno-mass spectrometry method for preclinical bioanalysis for IgG1 mAb

An immuno-mass spectrometry-based universal method for IgG1 mAb bioanalysis in preclinical phase successfully established using the Thermo Scientific SMART Digest IA Av Magnetic Kit, Thermo Scientific CaptureSelect Human IgG-Fc PK Biotin Conjugate, and SILuMAB K1 - Stable Isotope Labeled Universal mAb Standard. This method enjoyed the benefit of a fast simple workflow, high sensitivity, and method universality, and was also able to monitor the structural integrity while quantifying the mAb in vivo.

Nucleic-based bioanalysis

As nucleic acid therapeutics, such as mRNA, oligonucleotides, and gene therapies, continue to reshape modern medicine, analytical development teams face new challenges in sensitivity, specificity, and structural characterization.​

 

Thermo Fisher Scientific supports nucleic acid-based bioanalysis with a comprehensive suite of chromatography and mass spectrometry solutions, purpose-built to meet the complex demands of these modalities. From impurity profiling to intact mass analysis and metabolite identification, our platforms deliver the data quality and confidence required across the drug development pipeline​.

Sensitive quantitation of antisense oligonucleotides in plasma using high-resolution, accurate-mass (HRAM) mass spectrometry

As antisense oligonucleotide (ASO) therapeutics gain momentum, the need for reliable, high-sensitivity quantitation methods are growing. This application note demonstrates an IPRP-LC-HRAM-MS workflow using the Thermo Scientific Vanquish Horizon UHPLC and Thermo Scientific Orbitrap Exploris 120 MS for accurate ASO analysis in human plasma.

Automated sample preparation for protein quantitation

Automated sample preparation is a valuable tool in the bioanalysis of proteins because it ensures consistency and reproducibility of the digestion, increases efficiency and throughput, enhances precision and accuracy, saves time, and preserves sample integrity. By minimizing human error and handling multiple samples simultaneously, automation significantly improves the quality and reliability of protein quantitation, making it a critical component in modern bioanalytical workflows.

A fast and simple immuno-mass spectrometry method for preclinical bioanalysis for IgG1 mAb

An automated Protein digest for IgG1 mAb method was assessed for linearity, accuracy and precision. Compared with traditional immuno-mass spectrometry, this workflow avoids frequent sample transferring, protein denaturation, reduction/alkylation, and the subsequent solid-phase extraction step, shortening the original 2-day protocol to 4 hours with minimal hands-on preparation.

For Research Use Only. Not for use in diagnostic procedures.