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Thermo Fisher Scientific provides tailored solutions for precise and reliable analysis of protein therapeutic analysis. Our comprehensive range of applications ensures accurate identification, purity determination, quality assessment, and strength analysis. For detailed information on these specific applications, please refer to the sections below.
Complete protein characterization is a vital process that involves analyzing the composition, structure, and behavior of proteins. Through advanced techniques like mass spectrometry and bioinformatics, scientists can uncover valuable insights about proteins, including their amino acid composition, molecular weight, and post-translational modifications.
This knowledge has significant applications in fields such as drug discovery, biotechnology, personalized medicine, and improving food quality and nutrition. Overall, complete protein characterization is essential for understanding proteins and harnessing their full potential.
Bottom-up protein characterization poses several challenges and requires effective solutions to achieve accurate and comprehensive analysis. This approach involves digesting proteins into smaller peptides for analysis, which can lead to issues such as peptide complexity, dynamic range limitations, and the need for robust data analysis tools.
However, advancements in mass spectrometry, sample preparation techniques, and bioinformatics have provided solutions to overcome these challenges and enable in-depth bottom-up protein characterization. We propose a more efficient method that leverages Thermo Scientific Orbitrap-based LC-HRAM MS platforms for the comprehensive characterization and monitoring of protein therapeutics.
Top/middle-down protein characterization methods face unique challenges that require effective solutions for accurate and comprehensive analysis. These approaches involve analyzing intact or partially fragmented proteins, which can be challenging due to their size, complexity, and heterogeneity.
The challenges include limited fragmentation efficiency, low sensitivity, and difficulties in data interpretation. However, advancements in mass spectrometry instrumentation, fragmentation techniques, and bioinformatics have paved the way for innovative solutions in top-down and middle-down protein characterization.
For Research Use Only. Not for use in diagnostic procedures.