pSilencer™ 2.1-U6 puro Vector Map

pSilencer™ 2.1-U6 puro Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.

pSilencer™ 2.1-U6 puro Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

T7 primer: 5'-TAATACGACTCACTATAGGG-3'

2.0rev primer: 5'-AGGCGATTAAGTTGGGTA-3'




pSilencer™ 2.1-U6 puro Restriction Sites (Vector Size: 4455 bp)

Enzymes with single Restriction Sites

Site Enzyme
3586AccI
1250AflIII
3416AgeI
2143AhdI
1666AlwNI
3365AvrII
461BanHI
3387BmtI
4204BsaBI
3533BsiWI
4293BsmI
3390BspMI
3611BstEII
794EcoRI
399HindIII
4305HpaI
4292MfeI
3852MscI
3383NjeI
1250PciI
3061PflMI
759PmlI
4325PsiI
3401PstI
3593RsrII
3691SacII
3585SalI
1134SapI
3401SbfI
2623ScaI
3318SfiI
2947SspI
3519Tth111I
4193XbaI
2742XmnI

Non-Cutting Enzymes:

Enzyme
AarI
Acc65I
AfeI
AflII
AleI
AloI
AloI'
ApaI
AscI
AsiSI
BaeI
BaeI'
BbsI
BbvCI
BclI
BglII
BlpI
BmgBI
Bpu10I
BsgI
BspEI
BsrGI
BstBI
BstXI
BstZ17I
Bsu36I
BtrI
ClaI
EcoICRI
EcoNI
EcoRV
FalI
FalI'
FseI
FspAI
KpnI
MluI
NaeI
NgoMIV
NotI
NruI
PacI
PmeI
PpuMI
PshAI
PspOMI
PsrI
PsrI'
SacI
SanDI
SgfI
SgrAI
SnaBI
SpeI
SrfI
SwaI
TaqII'
XcmI
XhoI