ProcartaPlex 免疫测定

Invitrogen ProcartaPlex检测是基于抗体和微球的多重免疫检测技术，基于Luminex xMAP平台，可在单孔中仅需6.3–50 µL血浆、血清、细胞培养上清液或其他体液样本，即可同时检测并定量多达80种蛋白质靶标。该技术覆盖600余种靶标，包括细胞因子、趋化因子、生长因子及其他蛋白质，适用于人、小鼠、大鼠、非人灵长类、犬类、猪等多种物种。

广泛的预混panel（2至80重）和可定制panel，ProcartaPlex检测为炎症、免疫学、细胞因子分析、肿瘤学及传染病等研究领域提供高度灵活的解决方案。其准确性、灵敏度、可重复性和多功能性，助力研究人员深入解析复杂生物学过程。

ProcartaPlex多重检测采用96孔或384孔板格式，为生物标志物分析与疾病研究提供高通量、经济高效且省时的方案。

获取报价

了解ProcartaPlex检测原理

ProcartaPlex检测形式

预混panels

选择经过生物学验证的疾病相关检测组合，通过严格的兼容性、交叉反应和干扰测试确保性能与可重复性。

搜索预混panels

Mix & Match

按需设计定制检测组合，我们提供优化的定制方案。ProcartaPlex系统支持90%以上分析物的灵活组合。

创建专属panel

单重检测试剂盒

自行混合靶标构建检测组合，搭配ProcartaPlex基础试剂盒运行实验。

搜索单重检测试剂盒

双报告panels

新品上市 首个商业化ProcartaPlex双报告检测，可同时定量同一微球上最多8种信号蛋白的磷酸化与总蛋白。

了解更多

为什么选择 ProcartaPlex 检测？

灵活性

90%以上的检测可相互组合，支持创建高度个性化、多至80重的检测panel。

可重复的结果

ISO 9001认证设施生产，严格质量控制确保批次一致性。

可扩展性

不同规模检测panel与单重试剂盒数据高度可比。

高特异性与灵敏度

所有检测均经过特异性、天然内源蛋白检测及兼容性验证。

节省资源

相比单重检测，样本需求更少、操作时间更短，显著降低成本。

免费分析软件

通过赛默飞Connect平台免费使用ProcartaPlex Analyst App，含1TB云存储。

与QuantiGene Plex基因表达检测联合使用

ProcartaPlex免疫分析数据

Luminex xMAP INTELLIFLEX 上与 Luminex 200 和 FLEXMAP 3D 上 ProcartaPlex 检测性能的比较

(A) 人类 IL-8 标准曲线数据显示 INTELLIFLEX 与其他 Luminex 仪器结果类似。 INTELLIFLEX 上的采集是在低 PMT 模式 (LPMTLX) 下进行是为了与 LX200 进行比较；在高 PMT 模式 (HPMTFM) 下进行是为了与 FLEXMAP 3D 进行比较。(B) 对于从各种样本（未刺激和已刺激 PBMC）中获得的人类 IL-8 总蛋白测量结果，当数据是在 INTELLIFLEX 上采集时，与在 Luminex 200 和 FLEXMAP 3D 上采集相比，具有出色的相关性和一致性。

ProcartaPlex quality control standards

All preconfigured multi-analyte reagent panels are extensively tested for combinability, interference, and cross-reactivity and go through stringent quality control standards.

Specification	Description
Specificity	Proof of endogenous, native protein detection in specific samples
Sensitivity	Detection of relevant native protein levels
Precision	Inter-assay CV: <15%, Intra-assay CV: <15%
Linearity of dilution	Linear results over the range of the assay
Lot-to-lot consistency	Each new batch is calibrated against the reference standard lot
Dynamic range	Large dynamic range of all ProcartaPlex assays

Table 1. Cross-reactivity testing of analytes in ProcartaPlex preconfigured panels. Only a small subset of analytes are shown here.

Figure 1. Scalability data to demonstrate reproducibility between ProcartaPlex panels of different size. Comparison of protein expression data from 42 human blood samples analyzed using the ProcartaPlex Human Immune Monitoring Panel, 65-Plex and the ProcartaPlex Human Immune Response Panel, 80-Plex. A selection of analytes is shown here. Similar results are obtained when investigating other analytes.

Figure 2. Data illustrates the high correlation between large and small-scale multiplex assays when using the ProcartaPlex assays. Regression analysis comparing the ProcartaPlex Chemokine Panel 2 (9-plex) and the ProcartaPlex Human Immune Response Panel, Immune Response Panel (80-plex) yielded an R² value >0.9. (0.99 for MCP-4, 0.93 for CCL1)

Figure 3. Workflow of ProcartaPlex multiplex immunoassay

ProcartaPlex multiplex immunoassays for the Luminex platform are performed in a similar way to traditional ELISAs but with the ability to quantitate up to 80 targets simultaneously in a single well. The steps are similar, with the exception that antibody-coated beads are used to capture the analyte, instead of having capture antibodies attached directly to the plate. Each kit provides standards of known concentration so that a standard curve can be established. After incubation on a shaker, the beads are washed by putting the 96-well plate on a flat magnet for 30 seconds, after which the fluid is discarded by flicking the wells or by using an automated plate washer. The magnet is removed, and the beads are resuspended in the detection antibody. Another incubation and wash are followed by the addition of streptavidin–R-phycoerythrin (SAPE). The beads are then washed and are ready to analyze. Streamline your data analysis with the ProcartaPlex Analysis App.