Firefly Luciferase Assays and Vectors | Thermo Fisher Scientific

We offer Luciferase reporter reagents, multiple cloning site vectors, and flash, glow and dual-spectral luciferase assay kits based on the intracellularly expressed firefly luciferase. Firefly luciferase is a 60kDa protein produced in nature by several species of the Lampyridae family of beetles which includes the genera Photinus and Luciola. Bioluminescent signal from firefly luciferase originates from the oxidation of D-luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression.

Choose the right Firefly luciferase assay for your application

	Order now	Order now	Order now
	Pierce Firefly Luciferase Flash Assay Kits	Pierce Firefly Luciferase Glow Assay Kits	Pierce Firefly Luc One-Step Glow Assay Kits
Spectral Type	Single-spectral	Single-spectral	Single-spectral
Assay Type	Flash-type	Glow-type	Flash-type
Luciferase	Firefly	Firefly	Firefly
Description	Flash-type assays that provide an intense but short burst of light for maximum sensitivity May require the use of a luminometer with reagent injectors for the greatest signal strength and reduced variability	Glow-type assays that provide a less intense but longer-duration signal that is amenable to higher sample throughput Do not require reagent injectors	Automation friendly – amenable to high throughput assays One-step, mix-and-read protocol; no additional reagents required Glow-type assays that provide a less intense but longer-duration signal that is amenable to higher sample throughput Do not require reagent injectors
Average Half-Life	30 min	>1 hr	>1 hr

The Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

The Thermo Scientific Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.

Red firefly luciferase reporter assay vectors

Firefly luciferase reaction

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仅供科研使用，不可用于诊断目的。