PCR cleanup before Sanger sequencing

A one-step enzymatic clean-up reagent

Applied Biosystems™ CleanSweep™ PCR Purification Reagent is an easy-to-use, one-tube, single-step enzymatic clean-up reagent. CleanSweep PCR Purification Reagent dephosphorylates unincorporated nucleotides and digests unused primers to help ensure accurate downstream analysis of sequencing or single nucleotide polymorphism (SNP) data.

Your benefits:

• Single pipette step, simplified protocol—enzymes are provided in a single tube and one pipetting step is used to prepare the reaction, helping to ensure reliable results with reduced variability

• Efficient yield and turnaround time—equivalent performance to leading enzymatic PCR purification reagents

• Automation-friendly protocol—ability to be used in high-throughput or automated applications

• Economical—very cost-effective, one-step enzymatic PCR purification reagent offering robust results

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PCR cleanup before Sanger sequencing

Why is a clean template for your Sanger sequencing reaction important? And what are the ways to get it without losing too much precious sample? Find out today.

Read the full blog post here

 

So how do you perform this PCR clean up step and what are your options?

FREE
SAMPLE

Improve your sample sequencing results with this new one-step enzymatic PCR cleanup kit


Try a free CleanSweep reagent 20 rxn sample**.
Request sample ›

Save
50%

Replace your current PCR cleanup technology with our new product


Try CleanSweep reagent 100 rxn and save 50%***. Use promotion code RCSP50.
Get it now ›

**No purchase necessary. This promotion is available to life science professionals who are end-user customers in Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Holland, Hungary, Ireland, Italy, Luxemburg, Norway, Poland, Portugal, Slovakia, South Africa, Spain, Sweden, Switzerland and the UK. Sample requests must be received by December 31, 2016, or until sample supplies are depleted, whichever comes first. Sample size: 20 reactions. Limit one sample per laboratory. Please allow 3 weeks for delivery within Europe. Cannot be combined with other discounts or promotions. By requesting the free sample Customer warrants that he/she is not prohibited from by employment, law or any code of conduct or similar document from accepting the free sample and, where necessary, that his/her employer has consented thereto. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

***This promotion is open to customers in Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Hungary, Italy, The Netherlands, Norway, Poland, Portugal, Slovakia, South Africa, Spain, Sweden, Switzerland and the United Kingdom only. Discount will apply to qualifying orders of CleanSweep™ PCR Purification Reagent, catalog number A29895, received no later than June 30, 2016, or until promotional supplies are depleted, whichever comes first. Discount applies to the list price in effect at the time the order is received. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

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For Research Use Only. Not for use in diagnostic procedures.