With ULTRAhyb™
ULTRAhyb™
| Probe Type | Final Concentration in Hybridization Buffer |
|---|
| Radiolabeled RNA and DNA* probes | 106 cpm/ml |
| Nonisotopic DNA* probes labeled by enzymatic incorporation of a nonisotopically modified nucleotide | Up to 10 pM |
| Nonisotopic DNA* probes labeled by chemical labeling method (e.g., Ambion's BrightStar™ Psoralen-Biotin | 1 pM |
| Nonisotopic RNA probe | 0.1 nM |
| Oligonucleotide probes | Not recommended (use ULTRAhyb™-Oligo) |
| *Be sure to denature dsDNA probes prior to hybridization. |
With ULTRAhyb™-Oligo
ULTRAhyb™-Oligo™
| Probe Type | Final Concentration in Hybridization Buffer |
|---|
| Radiolabeled DNA oligonucleotide | 106 cpm/ml |
| Characteristic | Recommendation |
|---|
| Length | 26-45 |
| Tm value* | 78-90¨C |
| GC content | 47-62% |
| Specific activity | 5 x 106 cpm/pmol |
| Labeling method | 5' end labeling with [gamma-32P] ATP |
| *DNA oligonucleotides with a Tm outside of the range listed may require a different hybridization temperature that would have to be determined empirically. |
With Most Other Hybridization Solutions:
| Probe Type | Final Concentration in Hybridization Buffer |
|---|
| Radiolabeled RNA and DNA* probes | 1 x 106 to 5 x 106 cpm/ml |
| Nonisotopic RNA or DNA* probes | 0.1 nM |
| Oligonucleotide probes** | 1 x 106 to 5 x 106 cpm/ml |
*Be sure to denature dsDNA probes prior to hybridization.
**Note: Not all hybridization solutions are compatible with oligonucleotide probes. |