Do I need to remove Anti-Clumping Agent and Pluronic F-68 from the medium while growing Expi293 cells? How about during transfection of these cells?
You can have Anti-Clumping Agent and Pluronic F-68 in the medium while growing Expi293 cells. You need to remove Anti-Clumping Agent at least 2 passages prior to transfection of Expi293 cells because it interferes with transfection. You can add it back to the medium after transfection. Pluoronic F-68 can be present during transfection as it does not interfere with transfection.
How can I learn more about commercial licensing of Expi293F Cells (cGMP banked)?
To inquire about an Evaluation License, or Commercial Production License, or Service License, please email: outlicensing@thermofisher.com.
What is the term of the license agreement for Expi293F Cells (cGMP banked)?
As long as the license is not in breach, commercial use rights are granted in to perpetuity for the Commercial Production License. The term of the Commercial Service License is negotiated between the parties.
For the Expi293F Cells (cGMP banked), is there a pull-through royalty on final products or an annual maintenance fee?
Only a one-time license fee is required for a Commercial Production License, and it covers use during the clinical trial process and continues through the commercialization of one molecule.
Can I test the Expi293F Cells (cGMP banked) before committing to a Commercial License?
Yes. We offer an Evaluation Licensing Program, which allows you to purchase 1 vial of Expi293-F Cells (cGMP banked) for a one-time payment. A Limited Use Label License (LULL) accompanies each Evaluation License purchased from Life Technologies Corporation (LIFE) by the LIFE customer (Customer). The rights granted to a Customer with the purchase of an Evaluation License in the LULL include the following:
- Internal research use by Customer of such purchased Cell Line and the resulting molecules produced by such Cell Line
- Transfer of such Cell Line by Customer to a service provider (e.g., contract manufacturing organization (CMO) or contract research organization (CRO)) for Cell Line development and/or protein production solely for research and solely on behalf of and for the benefit of the Customer who purchased such Cell Line