Molecular characterization of hCTR1, the human copper uptake protein.
Authors Eisses John F; Kaplan Jack H;
JournalJ Biol Chem
PubMed ID12034741
'We have expressed hCTR1, the human copper transporter, in Sf9 cells using a baculovirus-mediated expression system, and we observed greatly enhanced copper uptake. Western blots showed that the protein is delivered to the plasma membrane, where it mediates saturable copper uptake with a K(m) of approximately 3.5 microm. We also ... More
Structure of the reovirus membrane-penetration protein, Mu1, in a complex with is protector protein, Sigma3.
Authors Liemann Susanne; Chandran Kartik; Baker Timothy S; Nibert Max L; Harrison Stephen C;
JournalCell
PubMed ID11832217
Cell entry by nonenveloped animal viruses requires membrane penetration without membrane fusion. The reovirus penetration agent is the outer-capsid protein, Mu1. The structure of Mu1, complexed with its ... More
Molecular characterization of major cat allergen Fel d 1: expression of heterodimer by use of a baculovirus expression system.
Fel d 1 is a major cat allergen inducing allergic rhinitis and asthma in sensitized individuals. It has a more complex structure when compared with other allergens and therefore expression of recombinant Fel d 1 has been considered a challenge. The present study shows for the first time that a ... More
A novel 43-kDa protein as a negative regulatory component of phenoloxidase-induced melanin synthesis.
AuthorsZhao M, Söderhäll I, Park JW, Ma YG, Osaki T, Ha NC, Wu CF, Söderhäll K, Lee BL,
JournalJ Biol Chem
PubMed ID15857824
The melanization reaction induced by activated phenoloxidase in arthropods is important in the multiple host defense innate immune reactions, leading to the sequestration and killing of invading microorganisms. This reaction ought to be tightly controlled because excessive formation of quinones and systemic hypermelanization are deleterious to the hosts, suggesting that ... More
Human MutY homolog, a DNA glycosylase involved in base excision repair, physically and functionally interacts with mismatch repair proteins human MutS homolog 2/human MutS homolog 6.
Authors Gu Yesong; Parker Antony; Wilson Teresa M; Bai Haibo; Chang Dau-Yin; Lu A-Lien;
JournalJ Biol Chem
PubMed ID11801590
Adenines mismatched with guanines or 7,8-dihydro-8-oxo-deoxyguanines that arise through DNA replication errors can be repaired by either base excision repair or mismatch repair. The human MutY homolog (hMYH), a DNA glycosylase, removes adenines from these mismatches. Human MutS homologs, hMSH2/hMSH6 (hMutSalpha), bind to the mismatches and initiate the repair on ... More
Sequence determinants in hypoxia-inducible factor-1alpha for hydroxylation by the prolyl hydroxylases PHD1, PHD2, and PHD3.
Authors Huang Jianhe; Zhao Quan; Mooney Sharon M; Lee Frank S;
JournalJ Biol Chem
PubMed ID12181324
Hypoxia-inducible factor (HIF) is a heterodimeric transcription factor induced by hypoxia. Under normoxic conditions, site-specific proline hydroxylation of the alpha subunits of HIF allows recognition by the von Hippel-Lindau tumor suppressor protein (VHL), a component of an E3 ubiquitin ligase complex that targets these subunits for degradation by the ubiquitin-proteasome ... More
Photolabeling of human and murine multidrug resistance protein 1 with the high affinity inhibitor [125I]LY475776 and azidophenacyl-[35S]glutathione.
Authors Qian Yue-Ming; Grant Caroline E; Westlake Christopher J; Zhang Da-Wei; Lander Peter A; Shepard Robert L; Dantzig Anne H; Cole Susan P C; Deeley Roger G;
JournalJ Biol Chem
PubMed ID12138119
Multidrug resistance protein 1 (MRP1/ABCC1) is an ATP-dependent transporter of structurally diverse organic anion conjugates. The protein also actively transports a number of non-conjugated chemotherapeutic drugs and certain anionic conjugates by a presently poorly understood GSH-dependent mechanism. LY475776is a newly developed (125)I-labeled azido tricyclic isoxazole that binds toMRP1 with high ... More
Purification and Characterization of the Schizosaccharomyces pombe Origin Recognition Complex. INTERACTION WITH ORIGIN DNA AND Cdc18 PROTEIN.
Authors Chuang Ray-Yuan; Chretien Louise; Dai Jianli; Kelly Thomas J;
JournalJ Biol Chem
PubMed ID11850415
The origin recognition complex (ORC) plays a central role in the initiation of DNA replication in eukaryotic cells. It interacts with origins of DNA replication in chromosomal DNA and recruits additional replication proteins to form functional initiation complexes. These processes have not been well characterized at the biochemical level except ... More