RNaseOUT™ 重组核糖核酸酶抑制剂
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RNaseOUT™ 重组核糖核酸酶抑制剂
Invitrogen™

RNaseOUT™ 重组核糖核酸酶抑制剂

RNaseOUT™ 重组核糖核酸酶抑制剂是胰腺型核糖核酸酶(如 RNase A)的强效非竞争性抑制剂,在多种应用中用于避免 RNA 降解。RNaseOUT™ 重组核糖核酸酶抑制剂是一种酸性蛋白,分子量为了解更多信息
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货号数量
107770195000 Units
货号 10777019
价格(CNY)
1,237.00
飞享价
Ends: 31-Dec-2025
2,300.00
共减 1,063.00 (46%)
Each
添加至购物车
数量:
5000 Units
请求批量或定制报价
价格(CNY)
1,237.00
飞享价
Ends: 31-Dec-2025
2,300.00
共减 1,063.00 (46%)
Each
添加至购物车
RNaseOUT™ 重组核糖核酸酶抑制剂是胰腺型核糖核酸酶(如 RNase A)的强效非竞争性抑制剂,在多种应用中用于避免 RNA 降解。RNaseOUT™ 重组核糖核酸酶抑制剂是一种酸性蛋白,分子量为 ∼52 kDa。RNaseOUT™ 可抑制 RNase A、RNase B 和 RNase C。

应用
cDNA 合成、RT-PCR 以及体外转录和翻译

来源
通过亲和色谱从表达克隆猪基因的大肠杆菌中纯化

性能和质量检测
SDS-PAGE 纯度、脱氧核糖核酸内切酶检测、蛋白浓度、比活度、通过 RT-PCR 评估的性能

单位定义
使用胞苷 2´, 3´ 环单磷酸 (cCMP) 作为底物,一单位可抑制 5 ng RNase A 的 50% 活性

单位反应条件
100 mM Tris-醋酸盐(pH 值 6.5)、1 mM EDTA、0.2 mM cCMP、2 µg RNase A,1 mL,25°C 下 0 至 10 min
仅供科研使用。不可用于诊断程序。
规格
最大浓度100 mM
产品线RNaseOUT™
数量5000 Units
Unit SizeEach
内容与储存
在 -20°C 下储存。避免产品暴露于频繁的温度变化。RNaseOUT™ 核糖核酸酶抑制剂需要 1 mM DTT 来维持活性。

除非产品文件中另有说明,否则产品可保证自购买日期起 6 个月内质量合格。

常见问题解答 (FAQ)

RNaseOUT 重组RNase抑制剂有什么作用?

该抑制剂可以防止在RNA准备过程中由于核糖核酸酶污染引起的靶RNA的降解。

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

What does RNaseOUT Recombinant RNase Inhibitor do?

The inhibitor acts as a safeguard against degradation of target RNA due to ribonuclease contamination of the RNA preparation.

What is the concentration of RNaseOUT Recombinant Ribonuclease Inhibitor in Units/microL?

RNaseOUT Recombinant Ribonuclease Inhibitor is provided at a concentration of 40 U/µL.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the temperature limitation of RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)?

RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019) unit reaction conditions are defined at 25 degrees C. The upper temperature limit for full functionality is 40 degrees C. The enzyme half-life is decreased as temperatures increase above 40 degrees C. There is some residual activity up to 50-55 degrees C but heating at 65 degrees C will inactivate the enzyme. As RNaseOUT can be used in various applications like cDNA synthesis, RT-PCR, and in vitro transcription, the recommendation is to follow the temperature settings required for the respective method.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.