What is the temperature limitation of RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)?
RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019) unit reaction conditions are defined at 25 degrees C. The upper temperature limit for full functionality is 40 degrees C. The enzyme half-life is decreased as temperatures increase above 40 degrees C. There is some residual activity up to 50-55 degrees C but heating at 65 degrees C will inactivate the enzyme. As RNaseOUT can be used in various applications like cDNA synthesis, RT-PCR, and in vitro transcription, the recommendation is to follow the temperature settings required for the respective method.