RNaseOUT™ Recombinant Ribonuclease Inhibitor - FAQs

该抑制剂可以防止在RNA准备过程中由于核糖核酸酶污染引起的靶RNA的降解。

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

The inhibitor acts as a safeguard against degradation of target RNA due to ribonuclease contamination of the RNA preparation.

RNaseOUT Recombinant Ribonuclease Inhibitor is provided at a concentration of 40 U/µL.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019) unit reaction conditions are defined at 25 degrees C. The upper temperature limit for full functionality is 40 degrees C. The enzyme half-life is decreased as temperatures increase above 40 degrees C. There is some residual activity up to 50-55 degrees C but heating at 65 degrees C will inactivate the enzyme. As RNaseOUT can be used in various applications like cDNA synthesis, RT-PCR, and in vitro transcription, the recommendation is to follow the temperature settings required for the respective method.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

RNAlater should not impact downstream PCR amplification as long as the sample has been properly cleaned before proceeding with DNA isolation, as stated in the RNAlater Stabilization Solution manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/7020M.pdf) on page 7.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

RNAlater Stabilization Solution stabilizes and protects cellular RNA in intact, unfrozen tissue samples, eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. In other words, it is an RNA preserving reagent.

RNaseOUT Recombinant Ribonuclease Inhibitor is a potent non-competitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used to avoid RNA degradation in a variety of applications such as cDNA synthesis, RT-PCR, and in vitro transcription and translation. RNAseOUT inhibits RNase A, RNAse B, and RNAse C. In other words, RNaseOUT Recombinant Ribonuclease Inhibitor is mainly used in an assay to prevent RNA degradation.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.