Grace's Insect Medium, unsupplemented, powder - FAQs

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2 个常见问题解答

Grace’s昆虫培养基中包含了哪些辅剂？为什么它们以单管形式提供？

补充培养基简称为TNM-FH。它包含以下成份（每500 mL）：

TC酵母粉：1.66 g，乳清蛋白水解物1.66 g，L-谷氨酰胺：0.3 g
这些添加剂能够帮助增加培养基的稳定性和延长保质期。请注意这些添加剂未经消毒，须经过滤灭菌（通过0.2 µm滤器）后方可加入。
TNM-FH未加入热灭活的10% FBS之前，不视为完全培养基。血清会提供额外的养分，同时也能够保护悬浮培养条件（即旋转器和振荡培养瓶）下的细胞免受流体压力的影响。

What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

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