Conversion of non-allosteric pyruvate kinase isozyme into an allosteric enzyme by a single amino acid substitution.
AuthorsIkeda Y, Tanaka T, Noguchi T
JournalJ Biol Chem
PubMed ID9252361
'Pyruvate kinase M1, a non-allosteric isozyme, was converted into an allosteric enzyme by replacement of an amino acid in the intersubunit contact. The substitution of Ala-398 with Arg resulted in the pronounced allosteric enzyme. The Hill coefficient and the substrate concentration giving one-half of Vmax for the mutant with respect ... More