'As a tetramer, acetylcholinesterase (AChE) is anchored to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses. We have previously shown that collagen Q (ColQ) anchors AChE at the neuromuscular junction. We have now cloned the gene PRiMA (proline-rich membrane anchor) encoding the AChE anchor ... More
Plaque production by the polyoma virus.
AuthorsDULBECCO R, FREEMAN G,
JournalVirology
PubMed ID13669362
Describes the original formulation of DMEM (Dulbecco’s Modified Eagle Medium), used for plaque production by the polyoma virus.
Interaction between GRIP and Liprin-alpha/SYD2 Is Required for AMPA Receptor Targeting.
Authors Wyszynski Michael; Kim Eunjoon; Dunah Anthone W; Passafaro Maria; Valtschanoff Juli G; Serra-Pagès Carles; Streuli Michel; Weinberg Richard J; Sheng Morgan;
JournalNeuron
PubMed ID11931740
Interaction with the multi-PDZ protein GRIP is required for the synaptic targeting of AMPA receptors, but the underlying mechanism is unknown. We show that GRIP binds to the liprin-alpha/SYD2 family of proteins that interact with LAR receptor protein tyrosine phosphatases (LAR-RPTPs) and that are implicated in presynaptic development. In neurons, ... More
Hypoxia and Nitric Oxide Treatment Confer Tolerance to Glucose Starvation in a 5'-AMP-activated Protein Kinase-dependent Manner.
Hypoxia is a critical event for higher organisms, and cells and tissues react by increasing the oxygen supply by vasodilatation, angiogenesis, and erythropoiesis and maintaining cellular energy by increasing glycolysis and inhibiting anabolic pathways. Stimulation of glycolysis has been regarded as the main response that increases energy production during hypoxia; ... More
Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces.
AuthorsLavasani M, Lu A, Thompson SD, Robbins PD, Huard J, Niedernhofer LJ
JournalMethods Mol Biol
PubMed ID23400434
Our lab developed and optimized a method, known as the modified pre-plate technique, to isolate stem/progenitor cells from skeletal muscle. This method separates different populations of myogenic cells based on their propensity to adhere to a collagen I-coated surface. Based on their surface markers and stem-like properties, including self-renewal, multi-lineage ... More
Neuromuscular electrical stimulation as a method to maximize the beneficial effects of muscle stem cells transplanted into dystrophic skeletal muscle.
AuthorsDistefano G, Ferrari RJ, Weiss C, Deasy BM, Boninger ML, Fitzgerald GK, Huard J, Ambrosio F
JournalPLoS One
PubMed ID23526927
Cellular therapy is a potential approach to improve the regenerative capacity of damaged or diseased skeletal muscle. However, its clinical use has often been limited by impaired donor cell survival, proliferation and differentiation following transplantation. Additionally, functional improvements after transplantation are all-too-often negligible. Because the host microenvironment plays an important ... More